Cao J, Wu L, Zhang H
Department of Ophthalmology, Third Clinical School of Medicine, Beijing Medical University.
Zhonghua Yan Ke Za Zhi. 1995 Mar;31(2):122-5.
The insulin-like growth factor I (IGF-I) levels in 67 vitreous samples taken from 57 patients with various proliferative retinal diseases and 10 normal subjects were detected by radioimmunoassay (RIA). The acid-ethanol-extracted (AEE) method was used to remove the IFG-binding protein before the detection. Our results revealed that IGF-I levels were increased to varying extents in these patients' vitreous. The IGF-I levels in the vitreous with proliferative diabetic retinopathy (PDR) and central retinal vein occlusion (CRVO) were significantly higher than those of the controls. In the PDR group, the increased IGF-I level in the vitreous was positively correlated with the level in serum. The increased IGF-I level in vitreous may play an important role in the development and pathogenesis of proliferative retinal diseases. Great differences were found between the native and the AEE treated vitreous, suggesting that the binding protein posed an obvious interference on the detection of IGF-I by RIA.
采用放射免疫分析法（RIA）检测了57例患有各种增生性视网膜疾病的患者及10名正常受试者的67份玻璃体样本中的胰岛素样生长因子I（IGF-I）水平。在检测前，使用酸乙醇提取法（AEE）去除IGF结合蛋白。我们的结果显示，这些患者玻璃体中的IGF-I水平有不同程度的升高。增生性糖尿病视网膜病变（PDR）和视网膜中央静脉阻塞（CRVO）患者玻璃体中的IGF-I水平显著高于对照组。在PDR组中，玻璃体中升高的IGF-I水平与血清中的水平呈正相关。玻璃体中升高的IGF-I水平可能在增生性视网膜疾病的发生和发病机制中起重要作用。在天然玻璃体和经AEE处理的玻璃体之间发现了很大差异，这表明结合蛋白对RIA检测IGF-I产生了明显干扰。
