Szymkowiak C H, Mons I, Gross W L, Kekow J
Department of Clinical Rheumatology, Medical University Lübeck, Germany.
J Immunol Methods. 1995 Aug 18;184(2):263-71. doi: 10.1016/0022-1759(95)00098-u.
Transforming growth factors beta (TGF beta s) show pleotrophic functions in vitro and in vivo. Biological effects depend on the cell type, the TGF beta isoform, and the availability of active TGF beta. Bioassays for TGF beta have not proven to be reliable tools in the measurement of TGF beta in human blood samples. Previously described sandwich ELISAs for measuring TGF beta are limited to single TGF beta isoforms and have not been evaluated for use in human sera or plasma. We describe a simple procedure to quantitate not only TGF beta 1 but also TGF beta 2 in human blood samples using commercially available antibodies. The sensitivity of the TGF beta 2 ELISA was 11 pg/ml. There was no crossreactivity between the TGF beta 1 and TGF beta 3 isoforms. High concentrations of TGF beta 1 and TGF beta 3 in spiked samples did not interfere with TGF beta 2 determination. TGF beta 2 recovery was highest in EDTA plasma (> 88%), and the intra- and interassay variance was 6% and 8.5% respectively. Applying the ELISA to human plasma specimens a significant percentage of TGF beta 2 (3.7 +/- 0.8 ng/ml) was found (about 50% of the TGF beta 1 content).
转化生长因子β（TGF-β）在体外和体内均表现出多效性功能。其生物学效应取决于细胞类型、TGF-β亚型以及活性TGF-β的可利用性。用于检测TGF-β的生物测定法尚未被证明是测量人类血液样本中TGF-β的可靠工具。先前描述的用于测量TGF-β的夹心ELISA仅限于单一的TGF-β亚型，且尚未评估其在人血清或血浆中的应用。我们描述了一种使用市售抗体不仅定量人血液样本中TGF-β1而且定量TGF-β2的简单方法。TGF-β2 ELISA的灵敏度为11 pg/ml。TGF-β1和TGF-β3亚型之间没有交叉反应性。加标样本中高浓度的TGF-β1和TGF-β3不干扰TGF-β2的测定。TGF-β2在EDTA血浆中的回收率最高（>88%），批内和批间变异分别为6%和8.5%。将ELISA应用于人血浆标本时，发现了相当比例的TGF-β2（3.7±0.8 ng/ml）（约为TGF-β1含量的50%）。
