Danielpour D, Roberts A B
Laboratory of Chemoprevention, National Cancer Institute, Bethesda, MD 20892, USA.
J Immunol Methods. 1995 Mar 27;180(2):265-72. doi: 10.1016/0022-1759(94)00322-n.
Transforming growth factors beta (TGF-beta) consist of a highly homologous family of 25 kDa dimers involved in a diverse array of biological functions. Progress in understanding the biology of the third isoform (TGF-beta 3) of this family has been limited by the absence of a quantitative assay for TGF-beta 3. Here we report the development of a sensitive and specific sandwich enzyme-linked immunosorbent assay (SELISA), which is based on an anti-TGF-beta mouse monoclonal IgG as the capture antibody and chicken anti-recombinant-hTGF-beta 3 IgY as the secondary antibody. This assay can quantitate TGF-beta 3 in complex biological fluids, with a detection limit of 2 pg and no cross-reactivity or interference with as high as 1000-fold molar excesses of either TGF-beta s 1, 2 or 1.2. This TGF-beta 3 SELISA is the first reported assay for the direct and sensitive quantitation of TGF-beta 3 in complex biological fluids.
转化生长因子β（TGF-β）由一个高度同源的25 kDa二聚体家族组成，参与多种生物学功能。由于缺乏针对TGF-β3的定量检测方法，该家族第三种异构体（TGF-β3）生物学特性的研究进展一直受限。在此，我们报告了一种灵敏且特异的夹心酶联免疫吸附测定法（SELISA）的开发，该方法以抗TGF-β小鼠单克隆IgG作为捕获抗体，鸡抗重组人TGF-β3 IgY作为二抗。该测定法能够对复杂生物体液中的TGF-β3进行定量，检测限为2 pg，对高达1000倍摩尔过量的TGF-β1、2或1.2均无交叉反应或干扰。这种TGF-β3 SELISA是首个报道的用于直接、灵敏定量复杂生物体液中TGF-β3的测定法。
