Kropf J, Schurek J O, Wollner A, Gressner A M
Department of Clinical Chemistry and Central Laboratory, Philipps University, Marburg, Germany.
Clin Chem. 1997 Oct;43(10):1965-74.
Development of a new, sensitive immunoassay for measuring transforming growth factor beta 1 (TGF-beta1) is described and compared with four commercially available TGF-beta1 immunoassays. Preanalytical conditions were evaluated. The nonlinearity found in serum or plasma is due to masking of TGF-beta1 by binding proteins in blood. Mixing TGF-beta1 with latency-associated peptide or alpha2-macroglobulin at physiological concentrations suppressed most of the TGF-beta1 signal. Plasma fibronectin showed no effect, even at concentrations exceeding its physiological range. Equilibrium concentrations computed from a model system confirmed the experimental results. Dilutional nonlinearity could be markedly reduced by an appropriately designed activation procedure that minimized the effects of reassociation between TGF-beta1 and its binding partners during restoration of a neutral pH. Plasma should be used for measuring TGF-beta1 in blood. Because serum TGF-beta1 is highly significantly correlated with the platelet count, probably most of the TGF-beta1 is released by platelet degranulation.
本文描述了一种用于测量转化生长因子β1（TGF-β1）的新型灵敏免疫测定方法，并将其与四种市售的TGF-β1免疫测定方法进行了比较。对分析前条件进行了评估。血清或血浆中发现的非线性是由于血液中的结合蛋白对TGF-β1的掩盖。在生理浓度下，将TGF-β1与潜伏相关肽或α2-巨球蛋白混合可抑制大部分TGF-β1信号。血浆纤连蛋白即使在浓度超过其生理范围时也没有影响。从模型系统计算出的平衡浓度证实了实验结果。通过适当设计的激活程序可以显著降低稀释非线性，该程序可在恢复中性pH值期间将TGF-β1与其结合伙伴重新结合的影响降至最低。应使用血浆来测量血液中的TGF-β1。由于血清TGF-β1与血小板计数高度显著相关，可能大部分TGF-β1是由血小板脱颗粒释放的。
