牛初乳样本中转化生长因子-β1（TGF-β1）和胰岛素样生长因子（IGF-1）的测定。

Determination of transforming growth factor-beta 1 (TGF-beta 1) and insulin-like growth factor (IGF-1) in bovine colostrum samples.

作者信息

Ginjala V, Pakkanen R

机构信息

Department of Biochemistry and Food Chemistry, University of Turku, Finland.

出版信息

J Immunoassay. 1998 May-Aug;19(2-3):195-207. doi: 10.1080/01971529808005480.

DOI:10.1080/01971529808005480

PMID:9682131

Abstract

The major growth factors in bovine colostrum are transforming growth factor-beta s (TGF-beta 1 and TGF-beta 2) and insulin-like growth factors (IGF-1 and IGF-2). Recently, TGF-beta 2 content of bovine colostrum was measured using a TGF-beta 2 specific ELISA (1) and now we have validated ELISAs for for bovine TGF-beta 1 and IGF-1. The concentrations of IGF-1 and TGF-beta 1 in the first milking after calving were 248-1850 ng/ml and 12.4-42.6 ng/ml, respectively, and they declined in correlation with total protein concentration to 27.0-101 ng/ml (IGF-1) and 0.80-3.49 ng/ml(TGF-beta 1) by the fifth milkings. The amount of TGF-beta 1 was on average 5.3 +/- 1.4% of that of TGF-beta 2 and there is a high correlation (r = 0.966) between the concentrations of these growth factors in the same samples. No free TGF-beta 1 form of could be detected.

摘要

牛初乳中的主要生长因子是转化生长因子-β（TGF-β1和TGF-β2）和胰岛素样生长因子（IGF-1和IGF-2）。最近，使用TGF-β2特异性酶联免疫吸附测定法（ELISA）测定了牛初乳中的TGF-β2含量(1)，现在我们已经验证了用于检测牛TGF-β1和IGF-1的ELISA方法。产犊后第一次挤奶时，IGF-1和TGF-β1的浓度分别为248 - 1850 ng/ml和12.4 - 42.6 ng/ml，到第五次挤奶时，它们与总蛋白浓度相关下降至27.0 - 101 ng/ml（IGF-1）和0.80 - 3.49 ng/ml（TGF-β1）。TGF-β1的量平均为TGF-β2的5.3±1.4%，并且在相同样品中这些生长因子的浓度之间存在高度相关性（r = 0.966）。未检测到游离形式的TGF-β1。