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Vector-hexamer PCR isolation of all insert ends from a YAC contig of the mouse Igh locus.从小鼠免疫球蛋白重链基因座的酵母人工染色体(YAC)重叠群中通过载体六聚体PCR分离所有插入末端。
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本文引用的文献

1
Identification of rat Tcrb-V8.2, 8.5, and 10 gene products by monoclonal antibodies.用单克隆抗体鉴定大鼠Tcrb-V8.2、8.5和10基因产物
Immunogenetics. 1993;37(4):305-8. doi: 10.1007/BF00187460.
2
Restriction-site PCR: a direct method of unknown sequence retrieval adjacent to a known locus by using universal primers.限制性酶切位点PCR:一种通过使用通用引物在已知基因座附近直接检索未知序列的方法。
PCR Methods Appl. 1993 May;2(4):318-22. doi: 10.1101/gr.2.4.318.
3
Low frequency of somatic mutation in beta-chain variable region genes of human T-cell receptors.人类T细胞受体β链可变区基因的体细胞突变频率较低。
Proc Natl Acad Sci U S A. 1985 Nov;82(22):7701-5. doi: 10.1073/pnas.82.22.7701.
4
Alternative splicing of murine T-cell receptor beta-chain transcripts.
Nature. 1986;322(6077):379-82. doi: 10.1038/322379a0.
5
A novel and rapid cloning method for the T-cell receptor variable region sequences.
Immunogenetics. 1991;34(3):174-8. doi: 10.1007/BF00205820.

A novel method for sequencing members of multi-gene families.

作者信息

Johnston S L, Strausbauch M, Sarkar G, Wettstein P J

机构信息

Department of Immunology, Mayo Foundation, Rochester, MN 55905, USA.

出版信息

Nucleic Acids Res. 1995 Aug 11;23(15):3074-5. doi: 10.1093/nar/23.15.3074.

DOI:10.1093/nar/23.15.3074
PMID:7659534
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC307155/
Abstract
摘要