Ledent E, Wasteson A, Berlin G
Department of Transfusion Medicine and Clinical Immunology, University Hospital, Linköping, Sweden.
Vox Sang. 1995;68(4):205-9. doi: 10.1111/j.1423-0410.1995.tb02573.x.
The platelet content of platelet-derived growth factor (PDGF), a mitogen stored in the alpha-granules, was studied during preparation and storage of platelet concentrates (PC) and compared to the growth-promoting activity of platelets, beta-thromboglobulin (beta-TG) and lactate dehydrogenase (LD). We compared PC prepared from platelet-rich plasma (PRP-PC; n = 10) and from buffy coat. Two different pre-preparation storage periods of the buffy coat were used: 4 h (BC-PC:4h; n = 10) and 24 h (BC-PC:24h; n = 5). The platelet content of PDGF and beta-TG was measured by a RIA technique and the growth-promoting activity by incorporation of 3H-thymidine in stimulated fibroblasts. The platelet content of PDGF, beta-TG and the growth-promoting activity of the platelets decreased in a similar way during preparation and storage of PRP-PC (31 +/- 2, 35 +/- 2 and 33 +/- 7%, respectively, at day 5 of storage; mean +/- SEM). The release of LD was minor (3.9 +/- 0.5% at day 5). At day 1 of storage the platelet content of PDGF was significantly better preserved in BC-PC:4h than in BD-PC:24h (88 +/- 2 and 81 +/- 3%, respectively; p = 0.03). Comparing BC-PC:4h and PRP-PC we found a significantly better preservation of PDGF in BC-PC:4h until day 3 of storage (80 +/- 2 and 75 +/- 1%, respectively at day 3; p = 0.046). In conclusion the preparation of PC according to the PRP method initially induces a higher loss of PDGF, and hence of the growth-promoting activity, than the BC method.
研究了储存在α颗粒中的促有丝分裂原血小板衍生生长因子（PDGF）的血小板含量，该研究在血小板浓缩物（PC）的制备和储存过程中进行，并与血小板、β-血小板球蛋白（β-TG）和乳酸脱氢酶（LD）的促生长活性进行比较。我们比较了由富血小板血浆制备的PC（PRP-PC；n = 10）和由白膜层制备的PC。白膜层使用了两种不同的制备前储存时间：4小时（BC-PC:4h；n = 10）和24小时（BC-PC:24h；n = 5）。通过放射免疫分析技术测量PDGF和β-TG的血小板含量，并通过在受刺激的成纤维细胞中掺入3H-胸腺嘧啶来测量促生长活性。在PRP-PC的制备和储存过程中，PDGF、β-TG的血小板含量以及血小板的促生长活性以相似的方式下降（储存第5天时分别为31±2、35±2和33±7%；平均值±标准误）。LD的释放量较小（储存第5天时为3.9±0.5%）。在储存第1天时，BC-PC:4h中PDGF的血小板含量比BD-PC:24h中保存得明显更好（分别为88±2和81±3%；p = 0.03）。比较BC-PC:4h和PRP-PC，我们发现直到储存第3天，BC-PC:4h中PDGF的保存明显更好（第3天时分别为80±2和75±1%；p = 0.046）。总之，与BC法相比，根据PRP法制备PC最初会导致更高的PDGF损失，从而导致促生长活性的损失。
