人参二醇皂苷Rb1、Rb2、Rb3、Rc和Rd的钙通道阻滞及抗自由基作用
[Calcium channel blockade and anti-free-radical actions of panaxadiol saponins Rb1, Rb2, Rb3, Rc, and Rd].
作者信息
Zhong G G, Sun C W, Li Y Y, Qi H, Zhao C Y, Jiang Y, Wang X M, Yang S J, Li H
机构信息
Department of Physiology, Norman Bethune Medical University, Changchun, China.
出版信息
Zhongguo Yao Li Xue Bao. 1995 May;16(3):255-60.
AIM
To identify the calcium channel blockade and anti-free-radical actions of panaxadiol saponins Rb1, Rb2, Rb3, Rc, and Rd.
METHODS
On ventricular myocardiocytes of Wistar rats, single channel activities of L, T, and B type Ca2+ channels were recorded with the cell-attached configuration of patch-clamp technic, and free radical contents were measured with electron spin resenance method.
RESULTS
Rb1, Rb2, Rb3, and Rc 200 mumol.L-1 shortened the open times, prolonged the close times, and reduced the openstate probabilities of calcium channels and 30 mumol.L-1 antagonized the increase of free radical content induced by xanthine 0.42 mmol.L-1-xanthine oxidase 5.3 nmol.L-1, but Rd in the same dose behaved none of the effects.
CONCLUSION
Rb1, Rb2, Rb3, and Rc had both the calcium channel blockade and anti-free-radical actions.
目的
鉴定人参二醇皂苷Rb1、Rb2、Rb3、Rc和Rd的钙通道阻滞作用及抗自由基作用。
方法
采用膜片钳技术的细胞贴附式记录Wistar大鼠心室肌细胞L、T和B型Ca2+通道的单通道活性，并用电子自旋共振法测定自由基含量。
结果
200μmol·L-1的Rb1、Rb2、Rb3和Rc缩短钙通道开放时间，延长关闭时间，降低开放概率，30μmol·L-1拮抗0.42mmol·L-1黄嘌呤-5.3nmol·L-1黄嘌呤氧化酶诱导的自由基含量增加，但相同剂量的Rd无上述作用。
结论
Rb1、Rb2、Rb3和Rc具有钙通道阻滞作用和抗自由基作用。
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