School of Kinesiology, Faculty of Health Sciences, Western University London, ON, Canada ; Ontario Ginseng Innovation and Research Consortium London, ON, Canada.
School of Kinesiology, Faculty of Health Sciences, Western University London, ON, Canada.
Front Pharmacol. 2014 Mar 14;5:43. doi: 10.3389/fphar.2014.00043. eCollection 2014.
Chronic ginseng treatments have been purported to improve cardiac performance. However reports of acute administration of ginseng on cardiovascular function remain controversial and potential mechanisms are not clear. In this study, we examined the effects of acute North American ginseng (Panax quinquefolius) administration on rat cardiac contractile function by using electrocardiogram (ECG), non-invasive blood pressure (BP) measurement, and Langendorff isolated, spontaneously beating, perfused heart measurements (LP). Eight-week old male Sprague-Dawley rats (n = 8 per group) were gavaged with a single dose of water-soluble American ginseng at 300 mg/kg body weight. Heart rate (HR) and BP were measured prior to and at 1 and 24 h after gavaging (ECG and BP). Additional groups were used for each time point for Langendorff measurements. HR was significantly decreased (ECG: 1 h: 6 ± 0.2%, 24 h: 8 ± 0.3%; BP: 1 h: 8.8 ± 0.2%, 24 h: 13 ± 0.4% and LP: 1 h: 22 ± 0.4%, 24 h: 19 ± 0.4%) in rats treated with water-soluble ginseng compared with pre or control measures. An initial marked decrease in left ventricular developed pressure was observed in LP hearts but BP changes were not observed in BP group. A direct inhibitory effect of North American ginseng was observed on cardiac contractile function in LP rats and on fluorescence measurement of intracellular calcium transient in freshly isolated cardiac myocytes when exposed to ginseng (1 and 10 μg/ml). Collectively these data present evidence of depressed cardiac contractile function by acute administration of North American ginseng in rat. This acute reduction in cardiac contractile function appears to be intrinsic to the myocardium.
慢性人参治疗据称可改善心脏功能。然而，关于人参急性给药对心血管功能的影响仍存在争议，潜在机制尚不清楚。在这项研究中，我们通过心电图（ECG）、非侵入性血压（BP）测量和 Langendorff 分离、自发跳动、灌注心脏测量（LP）检查了急性北美人参（Panax quinquefolius）给药对大鼠心脏收缩功能的影响。8 周龄雄性 Sprague-Dawley 大鼠（每组 8 只）经口给予水溶性美国人参 300mg/kg 体重。在灌胃前和灌胃后 1 和 24 小时测量心率（HR）和 BP（ECG 和 BP）。每个时间点还使用了其他组进行 Langendorff 测量。与预处理或对照措施相比，水溶性人参处理的大鼠 HR 明显降低（ECG：1 h：6 ± 0.2%，24 h：8 ± 0.3%；BP：1 h：8.8 ± 0.2%，24 h：13 ± 0.4%和 LP：1 h：22 ± 0.4%，24 h：19 ± 0.4%）。LP 心脏中观察到左心室发展压的初始明显下降，但在 BP 组中未观察到 BP 变化。当 LP 大鼠暴露于人参（1 和 10μg/ml）时，观察到北美人参对心脏收缩功能的直接抑制作用，以及对新鲜分离的心肌细胞内钙瞬变的荧光测量。总的来说，这些数据表明急性给予北美人参会导致大鼠心脏收缩功能下降。这种心脏收缩功能的急性下降似乎是心肌固有的。
