Godeny E K, Speicher D W, Brinton M A
Georgia State University, Biology Department, Atlanta 30303.
Virology. 1990 Aug;177(2):768-71. doi: 10.1016/0042-6822(90)90546-4.
Lactate dehydrogenase-elevating virus (LDV) is currently classified within the Togaviridae family. In an effort to obtain further information on the characteristics of this virus, we have begun to sequence the viral RNA genome and to map the virion structural protein genes. A sequence of 1064 nucleotides, which represents the 3' terminal end of the genome, was obtained from LDV cDNA clones. A 3' noncoding region of 80 nucleotides followed by two complete open reading frames (ORFs) were found within this sequence. The two ORFs were in different reading frames and overlapped each other by 11 nucleotides. One ORF encoded a protein of 170 amino acids and the other ORF, located adjacent to the 3' noncoding region of the viral genome, encoded a 114 amino acid protein. Thirty-three N-terminal residues were sequenced directly from purified LDV capsid protein, Vp1, and this amino acid sequence mapped to the ORF adjacent to the 3' noncoding region. The presence of overlapping ORFs and the 3' terminal map position of Vp1 indicate that LDV differs significantly from the prototype alpha togaviruses.
乳酸脱氢酶升高病毒（LDV）目前被归类于披膜病毒科。为了获取有关该病毒特征的更多信息，我们已开始对病毒RNA基因组进行测序并绘制病毒粒子结构蛋白基因图谱。从LDV cDNA克隆中获得了一段1064个核苷酸的序列，它代表基因组的3'末端。在该序列中发现了一个80个核苷酸的3'非编码区，其后是两个完整的开放阅读框（ORF）。这两个ORF处于不同的阅读框，并且彼此重叠11个核苷酸。一个ORF编码一个170个氨基酸的蛋白质，另一个ORF位于病毒基因组3'非编码区附近，编码一个114个氨基酸的蛋白质。从纯化的LDV衣壳蛋白Vp1直接测序了33个N末端残基，该氨基酸序列定位于与3'非编码区相邻的ORF。重叠ORF的存在以及Vp1的3'末端图谱位置表明LDV与原型α披膜病毒有显著差异。
