Litt M, Kramer P, Kort E, Fain P, Cox S, Root D, White R, Weissenbach J, Donis-Keller H, Gatti R
Department of Biochemistry, Oregon Health Sciences University, Portland 97201-3098, USA.
Genomics. 1995 May 1;27(1):101-12. doi: 10.1006/geno.1995.1011.
The CEPH consortium framework map of chromosome 11 is presented. The map was generated from CEPH family DNAs with 181 probe/enzyme combinations contributed by 20 laboratories. Seventy-seven of the loci are defined by microsatellite polymorphisms that can be typed by the PCR. A total of 42 loci have been placed on the map with likelihood support of at least 1000:1. The female, male, and sex-average maps extend for 179.6, 110.8, and 145.3 cM, respectively. The largest interval on the sex-average map is less than 11 cM, and the average distance between uniquely placed loci is 4 cM. The genotypic data obtained for map construction have been used to identify the positions of crossovers on the chromosomes of CEPH family children, allowing the localization of new markers without computationally intensive likelihood models and providing a basis for efficient extension of the linkage map to higher resolution.
本文展示了11号染色体的CEPH联盟框架图谱。该图谱由CEPH家族DNA构建而成，使用了来自20个实验室提供的181种探针/酶组合。其中77个位点由微卫星多态性定义，可通过聚合酶链反应（PCR）进行分型。共有42个位点被定位在图谱上，其似然支持度至少为1000:1。女性、男性和性别平均图谱分别延伸了179.6、110.8和145.3厘摩（cM）。性别平均图谱上最大的区间小于11厘摩，唯一定位位点之间的平均距离为4厘摩。用于构建图谱的基因型数据已被用于确定CEPH家族儿童染色体上交叉点的位置，无需使用计算量较大的似然模型即可定位新的标记，并为将连锁图谱高效扩展到更高分辨率提供了基础。
