日本慢生根瘤菌中的一个单一核糖体RNA基因区域。
A single rRNA gene region in Bradyrhizobium japonicum.
作者信息
Kündig C, Beck C, Hennecke H, Göttfert M
机构信息
Mikrobiologisches Institut, Eidgenössische Technische Hochschule, ETH-Zentrum, Zürich, Switzerland.
出版信息
J Bacteriol. 1995 Sep;177(17):5151-4. doi: 10.1128/jb.177.17.5151-5154.1995.
Abstract
Bradyrhizobium japonicum contains only a single rRNA (rrn) gene region, despite its comparatively large genome size of 8,700 kb. The nucleotide sequence revealed an organization of rRNA and tRNA genes that is frequently found in bacteria: 5'-rrs (16S rRNA)-ileT (tRNA(Ile))-alaT (tRNA(Ala))-rrl (23S rRNA)-rrf (5S rRNA)-3'. The 5' end of the primary transcript, one of the 16S rRNA processing sites, and the 5' end of the mature 16S rRNA were determined by primer extension. DNA hybridization experiments showed that the slowly growing Bradyrhizobium strains generally have only a single copy of the 16S rRNA gene, whereas the faster-growing Rhizobium species contain three rrs copies.
摘要
尽管日本慢生根瘤菌的基因组相对较大，达8700 kb，但它仅含有一个rRNA（rrn）基因区域。核苷酸序列显示了一种在细菌中常见的rRNA和tRNA基因组织形式：5'-rrs（16S rRNA）-ileT（tRNA（Ile））-alaT（tRNA（Ala））-rrl（23S rRNA）-rrf（5S rRNA）-3'。通过引物延伸确定了初级转录本的5'端、16S rRNA加工位点之一以及成熟16S rRNA的5'端。DNA杂交实验表明，生长缓慢的慢生根瘤菌菌株通常只有16S rRNA基因的单拷贝，而生长较快的根瘤菌物种则含有三个rrs拷贝。
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