杆状巴尔通体16S-23S rRNA基因间隔区的特征分析
Characterization of the 16S-23S rRNA intergenic spacer of Bartonella bacilliformis.
作者信息
Minnick M F, Strange J C, Williams K F
机构信息
Division of Biological Sciences, University of Montana, Missoula 59812-1002.
出版信息
Gene. 1994 May 27;143(1):149-50. doi: 10.1016/0378-1119(94)90622-x.
The 16S-23S intergenic spacer region from the ribosomal RNA (rRNA) operon of Bartonella bacilliformis was cloned and characterized. The spacer is 906 nucleotides (nt) in length and contains the genes encoding isoleucine-tRNA (tRNA(Ile)) and alanine-tRNA (tRNA(Ala)). The tRNA-encoding genes are separated by 122 nt and are centrally located in the 16S-23S spacer region, with approx. 300 flanking nt. Genes encoding tRNA(Ile) and tRNA(Ala) have 88.3 and 93.4% sequence identity, respectively, to the homologous genes of Rhodobacter sphaeroides.
对杆状巴尔通体核糖体RNA（rRNA）操纵子的16S - 23S基因间隔区进行了克隆和表征。该间隔区长906个核苷酸（nt），包含编码异亮氨酸 - tRNA（tRNA（Ile））和丙氨酸 - tRNA（tRNA（Ala））的基因。编码tRNA的基因被122个nt隔开，位于16S - 23S间隔区的中心位置，两侧约有300个nt。编码tRNA（Ile）和tRNA（Ala）的基因与球形红杆菌的同源基因的序列同一性分别为88.3%和93.4%。
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