空肠弯曲菌TGH9011（ATCC43431）rrnA操纵子的完整序列及结构

Complete sequences and organization of the rrnA operon from campylobacter jejuni TGH9011 (ATCC43431).

作者信息

Kim N W, Gutell R R, Chan V L

机构信息

Department of Microbiology, University of Toronto, Ontario, Canada.

出版信息

Gene. 1995 Oct 16;164(1):101-6. doi: 10.1016/0378-1119(95)00471-h.

DOI:10.1016/0378-1119(95)00471-h

PMID:7590296

Abstract

The rrnA ribosomal RNA (rRNA) operon of Campylobacter jejuni (Cj) TGH9011 (ATCC43431) was cloned and sequenced to completion. rRNAs were then characterized by primer extension and S1 nuclease mapping analysis. The secondary structure models of Cj 16S and 23S rRNAs were constructed, and the models were compared to the corresponding models from other eubacterial rRNA. The analysis presented a typical 5'-promoter-16S-tRNAs-23S-5S-terminator-3' prokaryotic rRNA operon structure. However, an unusual organization of the intercistronic tRNAs was observed where the two tRNAs, tRNA(Ala) and tRNA(Ile), were present in the order 5'-16S-tRNA(Ala)-tRNA(Ile)-23S-3', which is opposite of the typical 5'-16S-tRNA(Ile)-tRNA(Ala)-23S-3' structure observed in other bacteria.

摘要

空肠弯曲菌（Cj）TGH9011（ATCC43431）的rrnA核糖体RNA（rRNA）操纵子被克隆并测序完成。然后通过引物延伸和S1核酸酶图谱分析对rRNA进行了表征。构建了Cj 16S和23S rRNA的二级结构模型，并将这些模型与其他真细菌rRNA的相应模型进行了比较。分析呈现出典型的5'-启动子-16S-转运RNA-23S-5S-终止子-3'原核rRNA操纵子结构。然而，观察到顺反子间转运RNA存在异常排列，其中两个转运RNA，即丙氨酸转运RNA（tRNA(Ala)）和异亮氨酸转运RNA（tRNA(Ile)），以5'-16S-丙氨酸转运RNA-异亮氨酸转运RNA-23S-3'的顺序存在，这与在其他细菌中观察到的典型5'-16S-异亮氨酸转运RNA-丙氨酸转运RNA-23S-3'结构相反。