Nolte F S, Metchock B, Williams T, Diem L, Bressler A, Tenover F C
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA.
J Clin Microbiol. 1995 Jul;33(7):1804-6. doi: 10.1128/jcm.33.7.1804-1806.1995.
We compared penicillin MICs obtained with three different commercially available broth microdilution panels (MicroScan, Sensititre, and Pasco) with MICs obtained with reference microdilution panels for 20 well-characterized pneumococci with decreased susceptibilities to penicillin (7 resistant and 13 intermediate). All panels were supplemented with 2 to 5% lysed horse blood (LHB) prepared in-house. Additional supplements included fastidious inoculum broth (FIB) for MicroScan panels and commercially prepared LHB (Difco) for Pasco panels. The percentages of penicillin-resistant strains (MIC 2 micrograms/ml) detected by the different methods follow: MicroScan-FIB, 0; MicroScan-LHB 0; Pasco in-house LHB, 71; and Sensititre-LHB, 100. The percentages of intermediate strains (MIC = 0.1 to 1.0 micrograms/ml) detected by the different methods follow: MicroScan-FIB, 31; MicroScan-LHB 23; Pasco in-house LHB, 46; and Sensititre-LHB, 85. Difco LHB supplement failed to support the growth of 86% of the strains in the Pasco panels. Of the commercially available panels evaluated, only Sensititre, supplemented with LHB prepared in-house could reliably detect penicillin-resistant pneumococci.
我们将使用三种不同的市售肉汤微量稀释板（MicroScan、Sensititre和Pasco）获得的青霉素最低抑菌浓度（MIC）与使用参考微量稀释板获得的20株对青霉素敏感性降低的肺炎链球菌（7株耐药和13株中介）的MIC进行了比较。所有平板均添加了自制的2%至5%溶血马血（LHB）。其他补充剂包括用于MicroScan平板的苛求接种肉汤（FIB）和用于Pasco平板的市售LHB（Difco）。不同方法检测到的青霉素耐药菌株（MIC≥2微克/毫升）的百分比如下：MicroScan - FIB为0；MicroScan - LHB为0；Pasco自制LHB为71%；Sensititre - LHB为100%。不同方法检测到的中介菌株（MIC = 0.1至1.0微克/毫升）的百分比如下：MicroScan - FIB为31%；MicroScan - LHB为23%；Pasco自制LHB为46%；Sensititre - LHB为85%。Difco LHB补充剂未能支持Pasco平板中86%菌株的生长。在所评估的市售平板中，只有补充了自制LHB的Sensititre能够可靠地检测出青霉素耐药肺炎链球菌。
