Volant A, Nousbaum J B, Giroux M A, Roué-Quintin I, Metges J P, Férec C, Gouérou H, Robaszkiewicz M
CHU Morvan, Brest, France.
J Clin Pathol. 1995 Jun;48(6):531-4. doi: 10.1136/jcp.48.6.531.
To investigate the immunohistochemical expression of p53 protein in oesophageal squamous cell carcinomas and in dysplastic areas of the oesophageal mucosa surrounding the tumours.
Biopsy samples were obtained from 20 patients with an oesophageal squamous cell carcinoma. Blocks of the tumours and of the surrounding mucosa were immunostained with the monoclonal antibody DO-7.
Fourteen of the 20 carcinomas were positive for p53 (70%). The frequency of p53 overexpression increased with the differentiation of the tumour. Nine out of 13 dysplastic specimens were positive for p53 (69%): eight cases with severe dysplasia and one case with moderate dysplasia. No p53 immunostaining was detected in normal oesophageal epithelium. All p53 positive dysplastic specimens were taken from the mucosa adjacent to tumours that were also immunostained. In moderate dysplastic mucosa the p53 positive cells were located in the proliferative basal zone, whereas in severe dysplasia the immunostained cells increased in number and spread to upper cell layers of the epithelium.
This study supports the hypothesis that TP53 gene is frequently involved in the development of oesophageal squamous cell carcinoma and that p53 protein accumulation is an early event in human oesophageal carcinogenesis.
研究p53蛋白在食管鳞状细胞癌及肿瘤周围食管黏膜发育异常区域的免疫组化表达情况。
从20例食管鳞状细胞癌患者获取活检样本。肿瘤组织块及周围黏膜用单克隆抗体DO-7进行免疫染色。
20例癌组织中有14例p53呈阳性（70%）。p53过表达频率随肿瘤分化程度增加而升高。13例发育异常标本中有9例p53呈阳性（69%）：8例重度发育异常和1例中度发育异常。在正常食管上皮中未检测到p53免疫染色。所有p53阳性的发育异常标本均取自与免疫染色肿瘤相邻的黏膜。在中度发育异常黏膜中，p53阳性细胞位于增殖性基底区，而在重度发育异常中，免疫染色细胞数量增加并扩散至上皮细胞上层。
本研究支持以下假说，即TP53基因频繁参与食管鳞状细胞癌的发生发展，且p53蛋白积累是人类食管癌发生的早期事件。
