Lam K Y, Loke S L, Chen W Z, Cheung K N, Ma L
Department of Pathology, Queen Mary Hospital, Hong Kong.
Eur J Surg Oncol. 1995 Jun;21(3):242-7. doi: 10.1016/s0748-7983(95)91228-2.
We studied p53 overexpression in a series of 99 primary oesophageal squamous cell carcinomas (28 well-differentiated, 42 moderately-differentiated and 29 poorly-differentiated squamous cell carcinomas) from Chinese patients using the p53 protein specific mouse monoclonal antibody DO-7 on paraffin sections. The p53 protein was detected in 30% (30 cases) of the tumours. A significantly higher positive rate was noted in the poorly-differentiated tumours (11% for the well-differentiated, 31% for the moderately-differentiated and 48% for the poorly-differentiated tumours). In addition, strong positive p53 staining was identified only in the less differentiated tumour cells in the periphery of the tumour cell nests in all the cases and the expression was weaker in the better differentiated foci. The central keratinizing areas and the immediately adjacent tumour cells were always negative for p53. The adjacent normal oesophageal mucosa was all negative for p53 protein but the non-invasive dysplastic epithelium next to the tumours could also be strongly positive for p53 protein (four out of 14 cases in which the dysplastic epithelium adjacent to the tumour was adequately sampled). In two out of these four cases, the dysplastic epithelium showed staining for p53; even the adjacent invasive tumour was negative for p53. It is concluded that there is a strong relationship between p53 overexpression and tumour cell differentiation in oesophageal squamous carcinoma and overexpression of p53 can occur in non-invasive tumour cells.
我们使用p53蛋白特异性小鼠单克隆抗体DO-7，对来自中国患者的99例原发性食管鳞状细胞癌（28例高分化、42例中分化和29例低分化鳞状细胞癌）石蜡切片进行p53过表达研究。在30%（30例）的肿瘤中检测到p53蛋白。低分化肿瘤的阳性率显著更高（高分化肿瘤为11%，中分化肿瘤为31%，低分化肿瘤为48%）。此外，在所有病例中，仅在肿瘤细胞巢周边分化程度较低的肿瘤细胞中发现强阳性p53染色，而在分化较好的病灶中表达较弱。中央角化区域及其紧邻的肿瘤细胞p53始终为阴性。相邻的正常食管黏膜p53蛋白均为阴性，但肿瘤旁的非侵袭性发育异常上皮p53蛋白也可为强阳性（在对肿瘤旁发育异常上皮进行充分取样的14例病例中有4例）。在这4例中的2例中，发育异常上皮显示p53染色；甚至相邻的侵袭性肿瘤p53为阴性。结论是，食管鳞状癌中p53过表达与肿瘤细胞分化之间存在密切关系，并且p53过表达可发生在非侵袭性肿瘤细胞中。
