Randolph M F, Redys J J, Cope J, Morris K E
Am J Dis Child. 1976 Feb;130(2):171-2. doi: 10.1001/archpedi.1976.02120030061011.
One hundred sixty-eight children with upper respiratory infections were screened for beta-hemolytic streptococcal pharyngitis. A recently developed throat culture kit was processed in comparison with the conventional blood agar plate using a duplicate swab technique. Kits and plates were examined independently. Three individuals with no previous bacteriologic experience were allowed to interpret results of the culture kit after receiving instructions for approximately 15 minutes on three consecutive days. The interpretations of two of these individuals approached 95% accuracy, and those of the third individual surpassed this rate. The kit appears to be at least equal to the conventional blood agar plate in supporting the growth of beta-hemolytic streptococci. Moreover, its selective nature allows the nontechnical office staff to interpret results of the cultures promptly and accurately with a minimum of instruction.
对168名上呼吸道感染儿童进行了β-溶血性链球菌咽炎筛查。采用重复拭子技术,将一种新开发的咽喉培养试剂盒与传统血琼脂平板进行对比检测。试剂盒和培养平板独立检测。让三名此前没有细菌学经验的人员在连续三天接受约15分钟的指导后,对培养试剂盒的结果进行判读。其中两人的判读准确率接近95%,第三人的判读准确率超过了这一比例。该试剂盒在支持β-溶血性链球菌生长方面似乎至少与传统血琼脂平板相当。此外,其选择性特性使非技术办公人员只需最少的指导就能迅速、准确地判读培养结果。