黑寡妇毒液α- latrotoxins的基因结构、调控及进化
Gene structure, regulatory control, and evolution of black widow venom latrotoxins.
作者信息
Bhere Kanaka Varun, Haney Robert A, Ayoub Nadia A, Garb Jessica E
机构信息
Department of Biological Sciences, University of Massachusetts Lowell, MA, USA.
Department of Biology, Washington and Lee University, Lexington, VA, USA.
出版信息
FEBS Lett. 2014 Nov 3;588(21):3891-7. doi: 10.1016/j.febslet.2014.08.034. Epub 2014 Sep 12.
Abstract
Black widow venom contains α-latrotoxin, infamous for causing intense pain. Combining 33 kb of Latrodectus hesperus genomic DNA with RNA-Seq, we characterized the α-latrotoxin gene and discovered a paralog, 4.5 kb downstream. Both paralogs exhibit venom gland specific transcription, and may be regulated post-transcriptionally via musashi-like proteins. A 4 kb intron interrupts the α-latrotoxin coding sequence, while a 10 kb intron in the 3' UTR of the paralog may cause non-sense-mediated decay. Phylogenetic analysis confirms these divergent latrotoxins diversified through recent tandem gene duplications. Thus, latrotoxin genes have more complex structures, regulatory controls, and sequence diversity than previously proposed.
摘要
黑寡妇毒液含有α- Latrotoxin，因其会引发剧痛而声名狼藉。我们将33 kb的西方黑寡妇基因组DNA与RNA测序相结合，对α- Latrotoxin基因进行了表征，并在其下游4.5 kb处发现了一个旁系同源基因。这两个旁系同源基因均表现出毒腺特异性转录，并且可能通过类musashi蛋白在转录后受到调控。一个4 kb的内含子中断了α- Latrotoxin编码序列，而旁系同源基因3'UTR中的一个10 kb内含子可能导致无义介导的衰变。系统发育分析证实，这些不同的Latrotoxins是通过最近的串联基因复制而多样化的。因此，Latrotoxin基因具有比先前提出的更为复杂的结构、调控控制和序列多样性。
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