Diehl L J, Mathiason-Dubard C K, O'Neil L L, Obert L A, Hoover E A
Department of Pathology, Colorado State University, Fort Collins 80523, USA.
J Virol. 1995 Oct;69(10):6149-57. doi: 10.1128/JVI.69.10.6149-6157.1995.
Development of feline immunodeficiency virus (FIV) infection in cats as a small animal model for lentiviral immunodeficiency disease has been hampered by the prolonged and variable disease course following experimental infection. To address this issue, we generated high-titer, unselected FIV stocks by pooling plasma from cats acutely infected with a subgroup C FIV isolate designated CABCpadyOOC (FIV-C-PGammer). Subsequent infection with this virus pool resulted in rapidly progressive, fatal disease in greater than 50% of infected cats. Accelerated FIV disease was characterized by rapid and progressive CD4+ T-cell loss, lymphadenopathy, weight loss, lymphoid depletion, and severe thymic atrophy. Mortality and rate of disease progression were affected by the age of each cat at infection and whether the virus source animal was in the acute or chronic stage of infection. The rapid FIV disease syndrome was consistently associated with systemic lymphoid depletion, clinical disease, and susceptibility to opportunistic infections, analogous to accelerated and/or terminal HIV-1 infection. The results of this study demonstrate that FIV infection is a valid small animal model for lentiviral immunodeficiency disease.
猫免疫缺陷病毒（FIV）感染猫作为慢病毒免疫缺陷疾病的小动物模型，其实验感染后的病程延长且多变，阻碍了该模型的发展。为解决这一问题，我们通过汇集急性感染了一株C亚群FIV分离株（命名为CABCpadyOOC，即FIV-C-PGammer）的猫的血浆，制备了高滴度、未经筛选的FIV病毒储备液。随后用该病毒储备液感染猫，超过50%的感染猫出现了快速进展的致命疾病。加速发展的FIV疾病的特征为CD4+ T细胞迅速且进行性减少、淋巴结病、体重减轻、淋巴组织耗竭和严重的胸腺萎缩。死亡率和疾病进展速度受每只猫感染时的年龄以及病毒源动物处于感染急性期还是慢性期的影响。快速FIV疾病综合征始终与全身淋巴组织耗竭、临床疾病以及对机会性感染的易感性相关，类似于HIV-1感染的加速期和/或终末期。本研究结果表明，FIV感染是慢病毒免疫缺陷疾病有效的小动物模型。
