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微量滴定感染性测定法揭示的1型人类嗜T细胞病毒T淋巴细胞转化的时间进程和细胞因子依赖性

Time course and cytokine dependence of human T-cell lymphotropic virus type 1 T-lymphocyte transformation as revealed by a microtiter infectivity assay.

作者信息

Persaud D, Muñoz J L, Tarsis S L, Parks E S, Parks W P

机构信息

Department of Pediatrics, New York University Medical Center, New York 10016, USA.

出版信息

J Virol. 1995 Oct;69(10):6297-303. doi: 10.1128/JVI.69.10.6297-6303.1995.

Abstract

Human T-cell lymphotropic virus type 1 (HTLV-1) enhances the growth of T lymphocytes, allowing the generation of T-lymphocyte cell lines. This report describes a limiting-dilution assay system which uses low input numbers of HTLV-1-producing cells for generation of T-lymphocyte cultures. The HTLV-1 transformants generated with this assay system produced high levels of HTLV-1 p24 antigen and required exogenous cytokines for maintenance. Clonal populations of CD4- or CD8-positive HTLV-1 transformants were generated with transformation efficiency rates as high as 78%. An exogenous cytokine is necessary for HTLV-1 T-lymphocyte transformation, and cytokine dependence is the most likely outcome of infection and transformation. HTLV-1 T-lymphocyte transformation can occur in the presence of cytokines other than interleukin-2 (IL-2), such as IL-4 or IL-7. IL-4- or IL-7-dependent HTLV-1 transformants underwent T-lymphocyte mitogenesis in response to their homologous cytokines but proliferated best in the presence of IL-2. Since the receptors for IL-2, IL-4, and IL-7 share the IL-2 gamma chain, this component may be the common element in the signaling pathway for HTLV-1-associated transformation.

摘要

人类嗜T细胞病毒1型(HTLV-1)可促进T淋巴细胞的生长,从而产生T淋巴细胞系。本报告描述了一种有限稀释分析系统,该系统使用低输入数量的产生HTLV-1的细胞来生成T淋巴细胞培养物。用该分析系统产生的HTLV-1转化体产生高水平的HTLV-1 p24抗原,并且需要外源性细胞因子来维持。CD4或CD8阳性HTLV-1转化体的克隆群体以高达78%的转化效率产生。外源性细胞因子对于HTLV-1 T淋巴细胞转化是必需的,并且细胞因子依赖性是感染和转化最可能的结果。HTLV-1 T淋巴细胞转化可以在白细胞介素-2(IL-2)以外的细胞因子存在下发生,例如IL-4或IL-7。依赖IL-4或IL-7的HTLV-1转化体在其同源细胞因子的作用下经历T淋巴细胞有丝分裂,但在IL-2存在下增殖最佳。由于IL-2、IL-4和IL-7的受体共享IL-2γ链,该成分可能是HTLV-1相关转化信号通路中的共同元素。

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