Bian T H, Wang X F, Li X Y
Shanghai Institute of Materia Medica, Chinese Academy of Sciences.
Zhongguo Yao Li Xue Bao. 1995 Jul;16(4):315-8.
To study the effects of morphine on different lymphocytes and the influence of naloxone.
The proliferation rates of unmature, resting, and activated T-lymphocytes and B-lymphocytes were determined under various concentrations of morphine with or without naloxone in vitro.
Morphine 1 x 10(-10)-1 x 10(-6) mol L-1 enhanced concanavalin A (Con A)-induced splenic T-cell proliferation, and 1 mumol L-1 enhanced lipopolysaccharide (LPS)-induced splenic B-cell proliferation. Naloxone, which per se enhanced the T-cell proliferation, blocked the enhancing effects of morphine. Morphine (1 x 10(-10)-1 x 10(-5) mol L-1) had no influence on the proliferation of resting splenocytes and Con A-induced thymus cells. Morphine 1 mmol L-1 inhibited the proliferation of resting, LPS-induced splenocytes, and Con A-induced splenic and thymus cells. These inhibiting effects were not blocked by naloxone (50 mumol L-1).
Stimulating effect of morphine on activated T- and B-cells were mediated by opioid receptors and different opioid receptors existed during the differentiation and activation of lymphocytes. The inhibitory effects of morphine (1 mmol L-1) were not mediated by opioid receptors.
研究吗啡对不同淋巴细胞的作用及纳洛酮的影响。
在体外不同浓度吗啡存在或不存在纳洛酮的情况下，测定未成熟、静止和活化的T淋巴细胞及B淋巴细胞的增殖率。
1×10⁻¹⁰ - 1×10⁻⁶mol/L的吗啡增强了伴刀豆球蛋白A（Con A）诱导的脾T细胞增殖，1μmol/L的吗啡增强了脂多糖（LPS）诱导的脾B细胞增殖。本身能增强T细胞增殖的纳洛酮阻断了吗啡的增强作用。吗啡（1×10⁻¹⁰ - 1×10⁻⁵mol/L）对静止脾细胞和Con A诱导的胸腺细胞增殖无影响。1mmol/L的吗啡抑制静止、LPS诱导的脾细胞以及Con A诱导的脾细胞和胸腺细胞的增殖。这些抑制作用未被纳洛酮（50μmol/L）阻断。
吗啡对活化T细胞和B细胞的刺激作用由阿片受体介导，淋巴细胞分化和活化过程中存在不同的阿片受体。1mmol/L吗啡的抑制作用不是由阿片受体介导的。
