Nakajima H, Kobayashi M, Negishi T, Aono R
Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
Biosci Biotechnol Biochem. 1995 Jul;59(7):1323-5. doi: 10.1271/bbb.59.1323.
Escherichia coli strain JA300 grows in the presence of n-hexane, but not in the presence of cyclohexane. We isolated a 10.5-kb DNA fragment that provided cyclohexane tolerance on a multi-copy plasmid, from the chromosomal DNA of JA300. In this fragment, there were found C-terminal 10-amino acids truncated soxR (soxR') and soxS which control the superoxide response regulon genes. Characterization of subclones found that both the soxR' and overexpression of the soxS increased the levels of organic solvent tolerance in several E. coli strains.
大肠杆菌菌株JA300能在正己烷存在的情况下生长，但不能在环己烷存在的情况下生长。我们从JA300的染色体DNA中分离出一个10.5 kb的DNA片段，该片段在多拷贝质粒上赋予了对环己烷的耐受性。在这个片段中，发现了C端截短了10个氨基酸的soxR（soxR'）和控制超氧化物反应调节子基因的soxS。亚克隆的特性分析发现，soxR'和soxS的过表达都提高了几种大肠杆菌菌株对有机溶剂的耐受水平。
