Taguchi S, Ogawa T, Endo T, Momose H
Department of Biological Science and Technology, Science University of Tokyo, Chiba, Japan.
Biosci Biotechnol Biochem. 1998 Dec;62(12):2476-9. doi: 10.1271/bbb.62.2476.
An open reading frame (termed ORF-PR) encoding a metallothionein-like domain-including protein was found upstream of a previously identified Streptomyces chymotrypsin-type protease gene (sam-P20). Promoter and terminator activities of ORF-PR were detected using the promoterless Streptomyces tyrosinase gene as a reporter gene and expression of ORF-PR was supposed to occur before that of sam-P20 gene. Frameshift mutation analysis showed that the ORF-PR product might act as a repressive regulator of the sam-P20 gene.
在先前鉴定的嗜热栖热放线菌胰凝乳蛋白酶型蛋白酶基因（sam-P20）上游发现了一个开放阅读框（称为ORF-PR），其编码一种包含金属硫蛋白样结构域的蛋白质。使用无启动子的嗜热栖热放线菌酪氨酸酶基因作为报告基因检测了ORF-PR的启动子和终止子活性，并且推测ORF-PR的表达发生在sam-P20基因表达之前。移码突变分析表明，ORF-PR产物可能作为sam-P20基因的抑制性调节因子发挥作用。
