Goldberg J M, Friedman C I
Department of Gynecology, Ohio State University, Columbus, USA.
J Assist Reprod Genet. 1995 Feb;12(2):132-5. doi: 10.1007/BF02211382.
Estradiol (E2) induced ciliogenesis has been demonstrated in several mammalian species. There is no consensus as to what extent, if any, this occurs in humans. The aim of this study was to determine whether hormonal manipulation could limit deciliation and/or stimulate ciliogenesis using human tubal epithelium in vitro.
Experiment 1: Tubal epithelial organ explant cultures were established from nine women undergoing hysterectomy. Several segments from each tube were fixed immediately (in vivo control), the remainder were maintained in culture for one week. One group received no hormontal treatment (in vitro control). The others were supplemented with E2 2 ng/ml (low E2) or 10 ng/ml (high E2), Clomiphene citrate 300 ng/ml + low E2 (CC), and progesterone 300 ng/ml + low E2 (P4). All specimens were examined by scanning electron microscopy and the percent ciliated area determined. Experiment 2: Tubal epithelial cultures were established from an additional 11 patients. After 1 week in culture, half of the explants from each patient were supplemented with E2 15 ng/ml vs no E2 and maintained for another week. The specimens were examined as above, as well as with transmission electron microscopy (TEM).
Experiment 1: After 1 week in culture the mean percent ciliated areas in the in vitro control (24.8 +/- 17.9), low E2 (24.7 +/- 17.0), P4 (20.7 +/- 10.7), and CC (25.8 +/- 17.2) were approximately half the in vivo control (45.7 +/- 10.4), P = 0.005. The high E2 group (39.7 +/- 19.7) was significantly higher than the in vitro control, P = 0.008, but was not different from the in vivo control. Experiment 2: Both groups demonstrated a great reduction in ciliated area and were not significantly different from each other, E2 (8.3 +/- 10.8) and control (3.0 +/- 3.0). TEM failed to demonstrate ciliogenic precursors in either group.
High E2 was capable of preventing initial epithelial deciliation in vitro. Once deciliation started however, high E2 was unable to limit the process or induce ciliogenesis.
在几种哺乳动物中已证实雌二醇（E2）可诱导纤毛生成。但对于这种现象在人类中出现的程度（如果存在的话），尚无定论。本研究的目的是确定在体外使用人输卵管上皮进行激素处理是否能限制纤毛脱失和/或刺激纤毛生成。
实验1：从9名接受子宫切除术的女性建立输卵管上皮器官外植体培养物。每根输卵管的几个节段立即固定（体内对照），其余节段在培养中维持1周。一组不进行激素处理（体外对照）。其他组分别补充2 ng/ml E2（低E2）、10 ng/ml E2（高E2）、300 ng/ml枸橼酸氯米芬 + 低E2（CC）以及300 ng/ml孕酮 + 低E2（P4）。所有标本通过扫描电子显微镜检查并测定纤毛化区域百分比。实验2：从另外11名患者建立输卵管上皮培养物。培养1周后，每位患者的一半外植体补充15 ng/ml E2，另一半不补充E2，并再维持1周。标本如上述进行检查，同时进行透射电子显微镜（TEM）检查。
实验1：培养1周后，体外对照（24.8 ± 17.9）、低E2（24.7 ± 17.0）、P4（20.7 ± 10.7）和CC（25.8 ± 17.2）组的平均纤毛化区域百分比约为体内对照（45.7 ± 10.4）的一半，P = 0.005。高E2组（39.7 ± 19.7）显著高于体外对照，P = 0.008，但与体内对照无差异。实验2：两组的纤毛化区域均大幅减少，且彼此无显著差异，E2组（8.3 ± 10.8）和对照组（3.0 ± 3.0）。TEM未能在任何一组中显示纤毛生成前体。
高E2能够在体外防止初始上皮纤毛脱失。然而，一旦纤毛脱失开始，高E2无法限制这一过程或诱导纤毛生成。
