评估腺苷5'-三磷酸检测法作为检测显著菌尿症的筛查方法。
Evaluation of an adenosine 5'-triphosphate assay as a screening method to detect significant bacteriuria.
作者信息
Alexander D N, Ederer G M, Matsen J M
出版信息
J Clin Microbiol. 1976 Jan;3(1):42-6. doi: 10.1128/jcm.3.1.42-46.1976.
Abstract
The bioluminescent reaction of adenosine 5'-triphosphate (ATP) with luciferin and luciferase has been used in conjunction with a sensitive photometer (Lab-Line's ATP photometer) to detect significant bacteriuria in urine. This rapid method of screening urine specimens for bacteriuria was evaluated by using 348 urine specimens submitted to the clinical microbiology laboratory at the University of Minnesota Hospitals for routine culture using the calibrated loop-streak plate method. There was 89.4% agreement between the culture method and the ATP assay, with 7.0% false positive and 27.0% false negative results from the ATP assay using 10(5) organisms/ml of urine or greater as positive for significant bacteriuria and less than 10(5) organisms/ml as negative for significant bacteriuria.
摘要
腺苷5'-三磷酸（ATP）与荧光素和荧光素酶的生物发光反应已与灵敏光度计（Lab-Line的ATP光度计）结合使用，以检测尿液中的显著菌尿。通过使用348份尿液标本评估了这种快速筛查尿液标本中菌尿的方法，这些标本被提交到明尼苏达大学医院临床微生物实验室，采用校准的环划线平板法进行常规培养。培养法与ATP检测之间的一致性为89.4%，对于显著菌尿，ATP检测以每毫升尿液中10(5)个及以上生物体为阳性、低于每毫升10(5)个生物体为阴性时，假阳性率为7.0%，假阴性率为27.0%。
相似文献
1
Evaluation of an adenosine 5'-triphosphate assay as a screening method to detect significant bacteriuria.评估腺苷5'-三磷酸检测法作为检测显著菌尿症的筛查方法。
J Clin Microbiol. 1976 Jan;3(1):42-6. doi: 10.1128/jcm.3.1.42-46.1976.
2
Analysis of the disagreement between automated bioluminescence-based and culture methods for detecting significant bacteriuria, with proposals for standardizing evaluations of bacteriuria detection methods.基于自动生物发光法与培养法检测显著菌尿的差异分析，以及菌尿检测方法标准化评估的建议。
J Clin Microbiol. 1982 May;15(5):802-9. doi: 10.1128/jcm.15.5.802-809.1982.
3
Firefly luciferase ATP assay as a screening method for bacteriuria.萤火虫荧光素酶ATP检测法作为菌尿症的一种筛查方法。
J Clin Microbiol. 1983 Feb;17(2):218-24. doi: 10.1128/jcm.17.2.218-224.1983.
4
Detection of bacteriurea by bioluminescence.通过生物发光检测菌尿症。
Methods Enzymol. 1986;133:22-7. doi: 10.1016/0076-6879(86)33052-0.
5
Detection of bacteriuria by luciferase assay of adenosine triphosphate.通过三磷酸腺苷的荧光素酶测定法检测菌尿症。
J Clin Microbiol. 1975 Jan;1(1):1-8. doi: 10.1128/jcm.1.1.1-8.1975.
6
Screening of bacteria in urine using luciferin-luciferase assay of microbial ATP: a comparative study.
Ann Clin Biochem. 1982 Nov;19(6):416-20. doi: 10.1177/000456328201900605.
7
Rapid detection of bacteriuria in bacteriological routine laboratory: comparison between bioluminescence method and culture techniques.
Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol. 1983 Jul;254(4):515-20.
8
Application of bioluminescent urine screens in a tertiary care facility.生物发光尿液筛查在三级医疗机构中的应用。
Diagn Microbiol Infect Dis. 1986 Jan;4(1):1-10. doi: 10.1016/0732-8893(86)90050-7.
9
Performance characteristics of a new photometer with a moving filter tape for luminescence assay.一种带有可移动滤光带的新型光度计用于发光测定的性能特征。
Appl Environ Microbiol. 1977 Dec;34(6):720-4. doi: 10.1128/aem.34.6.720-724.1977.
10
Evaluation of a bioluminescence assay for the detection of bacteriuria.
Scand J Infect Dis. 1989;21(4):409-14. doi: 10.3109/00365548909167445.
引用本文的文献
1
Screening of urine cultures by three automated systems.通过三种自动化系统对尿培养进行筛查。
J Clin Microbiol. 1982 Mar;15(3):468-74. doi: 10.1128/jcm.15.3.468-474.1982.
2
Analysis of the disagreement between automated bioluminescence-based and culture methods for detecting significant bacteriuria, with proposals for standardizing evaluations of bacteriuria detection methods.基于自动生物发光法与培养法检测显著菌尿的差异分析，以及菌尿检测方法标准化评估的建议。
J Clin Microbiol. 1982 May;15(5):802-9. doi: 10.1128/jcm.15.5.802-809.1982.
3
Rapid processing of urine specimens by urine screening and the AutoMicrobic system.通过尿液筛查和自动微生物系统对尿液标本进行快速处理。
J Clin Microbiol. 1982 Oct;16(4):668-72. doi: 10.1128/jcm.16.4.668-672.1982.
4
Evaluation of a commercially available semi-automated bioluminescence system for bacteriuria screening.评估一种用于菌尿症筛查的市售半自动生物发光系统。
Eur J Clin Microbiol. 1982 Aug;1(4):223-7. doi: 10.1007/BF02019712.
5
Rapid detection of bacterial growth in blood cultures by bioluminescent assay of bacterial ATP.通过细菌ATP的生物发光测定法快速检测血培养中的细菌生长
J Clin Microbiol. 1983 Sep;18(3):521-5. doi: 10.1128/jcm.18.3.521-525.1983.
6
Comparison of the Lumac and Monolight systems for detection of bacteriuria by bioluminescence.Lumac系统和单光系统通过生物发光检测菌尿症的比较。
J Clin Microbiol. 1984 Oct;20(4):797-801. doi: 10.1128/jcm.20.4.797-801.1984.
7
Rapid detection of bacteriuria by laser nephelometry.通过激光散射比浊法快速检测菌尿症
J Clin Microbiol. 1984 Feb;19(2):240-4. doi: 10.1128/jcm.19.2.240-244.1984.
8
Rapid diagnosis of bacteraemia by measuring bacterial adenosine triphosphate in blood culture bottles using bioluminescence.
Med Microbiol Immunol. 1983;172(2):117-22. doi: 10.1007/BF02124512.
9
Urine screening with the MS-2.使用MS-2进行尿液筛查。
J Clin Microbiol. 1983 Jun;17(6):1061-5. doi: 10.1128/jcm.17.6.1061-1065.1983.
10
Firefly luciferase ATP assay as a screening method for bacteriuria.萤火虫荧光素酶ATP检测法作为菌尿症的一种筛查方法。
J Clin Microbiol. 1983 Feb;17(2):218-24. doi: 10.1128/jcm.17.2.218-224.1983.
本文引用的文献
1
The quantitative significance of bacteria visualized in the unstained urinary sediment.未染色尿沉渣中可见细菌的定量意义。
N Engl J Med. 1961 Sep 21;265:589-90. doi: 10.1056/NEJM196109212651207.
2
Bacteriuria and the diagnosis of infections of the urinary tract; with observations on the use of methionine as a urinary antiseptic.菌尿症与尿路感染的诊断；关于蛋氨酸作为尿路防腐剂应用的观察
AMA Arch Intern Med. 1957 Nov;100(5):709-14. doi: 10.1001/archinte.1957.00260110025004.
3
Asymptomatic infections of the urinary tract.无症状性尿路感染
Trans Assoc Am Physicians. 1956;69:56-64.
4
Ionic effects on firefly bioluminescence assay of red blood cell ATP.离子对红细胞ATP萤火虫生物发光测定的影响。
Anal Biochem. 1966 Nov;17(2):268-77. doi: 10.1016/0003-2697(66)90205-3.
5
An improved analysis of adenosine triphosphate by the luciferase method.通过荧光素酶法对三磷酸腺苷进行的改进分析。
Acta Chem Scand. 1968;22(6):1745-56. doi: 10.3891/acta.chem.scand.22-1745.
6
An automated procedure for the sensitive and specific determination of ATP.
Clin Chem. 1969 Jan;15(1):3-14.
7
Rapid detection of microorganisms in aerospace water systems.航空航天水系统中微生物的快速检测
Aerosp Med. 1968 Jan;39(1):14-6.
8
Analysis of adenosine 3',5'-monophosphate with luciferase luminescence.
Anal Biochem. 1970 May;35(1):91-7. doi: 10.1016/0003-2697(70)90014-x.
9
Use of the liquid scintillation spectrometer for determining adenosine triphosphate by the luciferase enzyme.使用液体闪烁光谱仪通过荧光素酶测定三磷酸腺苷。
Anal Biochem. 1969 Jun;29(3):381-92. doi: 10.1016/0003-2697(69)90323-6.
Zotero 插件