Alexander D N, Ederer G M, Matsen J M
J Clin Microbiol. 1976 Jan;3(1):42-6. doi: 10.1128/jcm.3.1.42-46.1976.
The bioluminescent reaction of adenosine 5'-triphosphate (ATP) with luciferin and luciferase has been used in conjunction with a sensitive photometer (Lab-Line's ATP photometer) to detect significant bacteriuria in urine. This rapid method of screening urine specimens for bacteriuria was evaluated by using 348 urine specimens submitted to the clinical microbiology laboratory at the University of Minnesota Hospitals for routine culture using the calibrated loop-streak plate method. There was 89.4% agreement between the culture method and the ATP assay, with 7.0% false positive and 27.0% false negative results from the ATP assay using 10(5) organisms/ml of urine or greater as positive for significant bacteriuria and less than 10(5) organisms/ml as negative for significant bacteriuria.
腺苷5'-三磷酸（ATP）与荧光素和荧光素酶的生物发光反应已与灵敏光度计（Lab-Line的ATP光度计）结合使用，以检测尿液中的显著菌尿。通过使用348份尿液标本评估了这种快速筛查尿液标本中菌尿的方法，这些标本被提交到明尼苏达大学医院临床微生物实验室，采用校准的环划线平板法进行常规培养。培养法与ATP检测之间的一致性为89.4%，对于显著菌尿，ATP检测以每毫升尿液中10(5)个及以上生物体为阳性、低于每毫升10(5)个生物体为阴性时，假阳性率为7.0%，假阴性率为27.0%。
