Lee M R, Ederer G M
J Clin Microbiol. 1977 Mar;5(3):290-2. doi: 10.1128/jcm.5.3.290-292.1977.
The rapid hippurate hydrolysis test was evaluated with the conventional test, using 17 group A streptococci, 9 non-enterococcal group D streptococci, 108 enterococcal group D streptococci, and 2 strains of Listeria monocytogenes. There was complete correlation between the rapid and conventional tests with all organisms except enterococcal group D. The rapid hippurate hydrolysis method was more sensitive with the enterococci; 95.4% were positive with the rapid method, and 9.3% were positive with the conventional method. Thin-layer chromatography (TLC) was performed on all isolates to determine if the end product of hydrolysis, glycine, was indeed present. The TLC results were in agreement with the rapid and conventional methods for group A streptococci, nonenterococcal group D streptococci, and L. monocytogenes. TLC results were in total agreement with the rapid hippurate hydrolysis test for the enterococcal group D isolates, thus verifying the accuracy of this more sensitive test. Trace amounts of glycine were found in the substrate, indicating the need for including an uninoculated substrate control as well as stock strains of group A and B beta-hemolytic streptococci (negative and positive controls, respectively) each time the rapid hippurate hydrolysis test is performed.
采用常规试验对快速马尿酸盐水解试验进行了评估，受试菌株包括17株A群链球菌、9株非肠球菌D群链球菌、108株肠球菌D群链球菌和2株单核细胞增生李斯特菌。除肠球菌D群外，快速试验与常规试验对所有受试菌株的检测结果完全一致。快速马尿酸盐水解方法对肠球菌更为敏感；快速法检测阳性率为95.4%，常规法检测阳性率为9.3%。对所有分离株进行了薄层色谱（TLC）分析，以确定水解终产物甘氨酸是否确实存在。TLC结果与A群链球菌、非肠球菌D群链球菌和单核细胞增生李斯特菌的快速试验及常规试验结果一致。TLC结果与肠球菌D群分离株的快速马尿酸盐水解试验结果完全一致，从而验证了这种更敏感试验的准确性。在底物中发现了痕量甘氨酸，这表明每次进行快速马尿酸盐水解试验时都需要设置未接种底物对照以及A群和B群β溶血性链球菌的标准菌株（分别为阴性和阳性对照）。
