Imamura N, Dake Y, Amemiya T
Department of Ophthalmology, Nagasaki University School of Medicine, Japan.
Life Sci. 1995;57(13):1317-23. doi: 10.1016/0024-3205(95)02075-t.
The wake-sleep rhythm is a typical circadian rhythm. It has been reported that severely disturbed wake-sleep rhythms are improved by the administration of vitamin B12. This study was designed to clarify whether vitamin B12 is universally related to the circadian rhythm. Three-week-old male Wistar Kyoto rats were fed a vitamin B12 deficient diet for 3 months. Then some of them were given intraperitoneal injections of vitamin B12. We counted phagocytized lamellar structures in the retinal pigment epithelium after the start of exposure to light in negative electron microscopic films. In vitamin B12 deficient rats, the maximum number of phagocytized lamellar structures in the retinal pigment epithelium appeared 3 hours after the start of light exposure, while in the control and vitamin B12 injected groups the peak occurred at 2 hours. This difference suggests that the retinal circadian rhythm is closely related to the level of vitamin B12.
觉醒-睡眠节律是一种典型的昼夜节律。据报道，严重紊乱的觉醒-睡眠节律可通过给予维生素B12得到改善。本研究旨在阐明维生素B12是否与昼夜节律普遍相关。将3周龄的雄性Wistar Kyoto大鼠喂以缺乏维生素B12的饮食3个月。然后给其中一些大鼠腹腔注射维生素B12。我们在负电子显微镜胶片中记录了光照开始后视网膜色素上皮中吞噬的层状结构数量。在缺乏维生素B12的大鼠中，视网膜色素上皮中吞噬的层状结构的最大数量在光照开始后3小时出现，而在对照组和注射维生素B12的组中，峰值出现在2小时。这种差异表明视网膜昼夜节律与维生素B12水平密切相关。
