Vanek-Krebitz M, Hoffmann-Sommergruber K, Laimer da Camara Machado M, Susani M, Ebner C, Kraft D, Scheiner O, Breiteneder H
Institute of General and Experimental Pathology, University of Vienna, Austria.
Biochem Biophys Res Commun. 1995 Sep 14;214(2):538-51. doi: 10.1006/bbrc.1995.2320.
The number of tree-pollen-allergic patients showing allergic reactions to apples, various vegetables and nuts is increasing. In this paper the molecular characterization of the major apple allergen, Mal d 1, is reported. The cDNA coding for Mal d 1 was cloned and sequenced. Its open reading frame codes for a protein of 159 amino acids with a predicted molecular mass of 17.7 kDa and a predicted pI of 5.9. Sequence comparison to Bet v 1, the major birch pollen allergen, revealed 64.5% identity on the amino acid level and 55.6% identity on the nucleic acid level. Recombinant Mal d 1 was expressed in the plasmid pMW 175 in E. coli BL 21 (DE3) and its immunological properties were tested. Crossreactivity with Bet v 1 was shown by inhibition assays.
对苹果、各种蔬菜和坚果出现过敏反应的树花粉过敏患者数量正在增加。本文报道了主要苹果过敏原Mal d 1的分子特征。编码Mal d 1的cDNA被克隆并测序。其开放阅读框编码一个由159个氨基酸组成的蛋白质，预测分子量为17.7 kDa，预测pI为5.9。与主要桦树花粉过敏原Bet v 1的序列比较显示，氨基酸水平上的同一性为64.5%，核酸水平上的同一性为55.6%。重组Mal d 1在大肠杆菌BL 21 (DE3)的质粒pMW 175中表达，并对其免疫特性进行了测试。抑制试验显示了与Bet v 1的交叉反应性。
