Iteğin M, Günay I, Loğoğlu G, Isbir T
Department of Biophysics, Cukurova University Medical Faculty, Balcalì-Adana, Türkey.
Bioelectromagnetics. 1995;16(3):147-51. doi: 10.1002/bem.2250160302.
In this study, we aimed to clarify the effects of chronically applied static magnetic field (200 Gauss) on specific ATPase activities and bioelectrical and biomechanical responses in isolated rat diaphragm muscle. The mean activities of Na(+)-K+ ATPase and Ca2+ ATPase determined from the diaphragm homogenates were significantly higher in the magnetic field exposed group (n = 20), but that of Mg2+ ATPase was nonsignificantly lower compared to the control group (n = 13). Resting membrane potential, amplitude of muscle action potential, and overshoot values (mean +/- SE) in the control group were found to be -76.5 +/- 0.6, 100 +/- 0.8, and 23.5 +/- 0.6 mV, respectively; these values were determined to be -72.8 +/- 0.4, 90.3 +/- 0.5, and 17.2 +/- 0.4 mV in the magnetic field-exposed group, respectively. The latency was determined to increase in the experimental group, and all the above-mentioned bioelectrical differences between the groups were significant statistically. Force of muscle twitch was found to decrease significantly in the magnetic field-exposed group, and this finding was attributed to the augmenting effect of magnetic field on Ca2+ ATPase activity. These results suggest that magnetic field exposure changes specific ATPase activities and, thence, bioelectrical and biomechanical properties in the rat diaphragm muscle.
在本研究中，我们旨在阐明长期施加静磁场（200高斯）对离体大鼠膈肌特定ATP酶活性以及生物电和生物力学反应的影响。从膈肌匀浆中测得的Na(+)-K+ ATP酶和Ca2+ ATP酶的平均活性在磁场暴露组（n = 20）中显著更高，但Mg2+ ATP酶的活性与对照组（n = 13）相比虽有降低但无统计学意义。对照组的静息膜电位、肌肉动作电位幅度和超射值（平均值±标准误）分别为-76.5±0.6、100±0.8和23.5±0.6 mV；在磁场暴露组中，这些值分别为-72.
