Makinistian L, Belyaev I
Department of Radiobiology, Cancer Research Institute, Biomedical Center, Slovak Academy of Sciences, Bratislava, Slovakia.
Department of Physics and Instituto de Física Aplicada (INFAP), Universidad Nacional de San Luis, Consejo Nacional de Investigaciones Científicas y Técnicas, Ejército de los Andes 950, 5700 San Luis, Argentina.
R Soc Open Sci. 2018 Feb 14;5(2):172095. doi: 10.1098/rsos.172095. eCollection 2018 Feb.
A thorough assessment of the static magnetic field (SMF) inside a CO incubator allowed us to identify non-negligible inhomogeneities close to the floor, ceiling, walls and the door. Given that incubator's shelves are made of a non-magnetic stainless steel alloy, we did not expect any important effect of them on the SMF. Surprisingly, we did find relatively strong distortion of the SMF due to shelves. Indeed, our high-resolution maps of the SMF revealed that distortion is such that field intensities differing by a factor of up to 36 were measured on the surface of the shelf at locations only few millimetres apart from each other. Furthermore, the most intense of these fields was around five times greater than the ones found inside the incubator (without the metallic shelves in), while the lowest one was around 10 times lower, reaching the so-called hypomagnetic field range. Our findings, together with a survey of the literature on biological effects of hypomagnetic fields, soundly support the idea that SMF inhomogeneities inside incubators, especially due to shelves' holes, are a potential source of confounding and variability in experiments with cell cultures kept in an incubator.
对二氧化碳培养箱内静磁场（SMF）的全面评估使我们能够识别出靠近地板、天花板、墙壁和门处不可忽略的不均匀性。鉴于培养箱的搁板由非磁性不锈钢合金制成，我们并未预期它们会对静磁场产生任何重大影响。令人惊讶的是，我们确实发现搁板会导致静磁场出现相对较强的畸变。实际上，我们的静磁场高分辨率地图显示，畸变程度如此之大，以至于在搁板表面仅相隔几毫米的位置测量到的场强相差高达36倍。此外，这些场中最强的大约是培养箱内（没有金属搁板时）场强的五倍，而最低的则低约10倍，进入了所谓的低磁场范围。我们的研究结果，连同对低磁场生物效应文献的调查，有力地支持了这样一种观点，即培养箱内的静磁场不均匀性，尤其是由于搁板的孔洞导致的，是在培养箱中进行细胞培养实验时潜在的混杂和变异性来源。
