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1
Ca2+/calmodulin-dependent nitric oxide synthase activity in the human cervix carcinoma cell line ME-180.人子宫颈癌细胞系ME-180中钙/钙调蛋白依赖性一氧化氮合酶活性
Biochem J. 1993 Jan 15;289 ( Pt 2)(Pt 2):357-61. doi: 10.1042/bj2890357.
2
Ca2+/calmodulin-dependent formation of hydrogen peroxide by brain nitric oxide synthase.脑一氧化氮合酶通过钙/钙调蛋白依赖性方式生成过氧化氢。
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3
Cytokine-activated endothelial cells express an isotype of nitric oxide synthase which is tetrahydrobiopterin-dependent, calmodulin-independent and inhibited by arginine analogs with a rank-order of potency characteristic of activated macrophages.细胞因子激活的内皮细胞表达一种一氧化氮合酶同工型，该同工型依赖四氢生物蝶呤、不依赖钙调蛋白，且被精氨酸类似物抑制，其抑制效力顺序具有活化巨噬细胞的特征。
Biochem Biophys Res Commun. 1991 Aug 15;178(3):823-9. doi: 10.1016/0006-291x(91)90965-a.
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Identification and characterization of a calmodulin-dependent nitric oxide synthase from GH3 pituitary cells.从GH3垂体细胞中鉴定和表征一种钙调蛋白依赖性一氧化氮合酶。
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Pineal nitric oxide synthase: characteristics, adrenergic regulation and function.
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Science. 1992 Feb 7;255(5045):721-3. doi: 10.1126/science.1371193.
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Purification of a Ca2+/calmodulin-dependent nitric oxide synthase from porcine cerebellum. Cofactor-role of tetrahydrobiopterin.
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Nitric oxide modulates pepsinogen secretion induced by calcium-mediated agonist in guinea pig gastric chief cells.一氧化氮调节豚鼠胃主细胞中钙介导的激动剂诱导的胃蛋白酶原分泌。
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10
Generation of superoxide by purified brain nitric oxide synthase.
J Biol Chem. 1992 Dec 5;267(34):24173-6.

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Biomed Res Int. 2017;2017:1309485. doi: 10.1155/2017/1309485. Epub 2017 Oct 18.
2
Reductive heme-dependent activation of the n-oxide prodrug AQ4N by nitric oxide synthase.一氧化氮合酶对氮氧化物前药AQ4N的还原性血红素依赖性激活作用。
J Med Chem. 2008 Aug 28;51(16):5118-20. doi: 10.1021/jm800496s. Epub 2008 Aug 6.
3
Tetrahydrobiopterin alters superoxide and nitric oxide release in prehypertensive rats.四氢生物蝶呤改变高血压前期大鼠体内超氧化物和一氧化氮的释放。
J Clin Invest. 1998 Apr 1;101(7):1530-7. doi: 10.1172/JCI650.
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Nitric oxide synthase activity in human lung cancer.人肺癌中的一氧化氮合酶活性
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Simultaneous expression of nitric oxide synthase and estrogen receptor in human breast cancer cell lines.一氧化氮合酶与雌激素受体在人乳腺癌细胞系中的同时表达。
Breast Cancer Res Treat. 1996;40(2):205-7. doi: 10.1007/BF01806216.
6
In search of a function for tetrahydrobiopterin in the biosynthesis of nitric oxide.寻找四氢生物蝶呤在一氧化氮生物合成中的作用。
Naunyn Schmiedebergs Arch Pharmacol. 1995 May;351(5):453-63. doi: 10.1007/BF00171035.
7
Nitric oxide synthase activity in human breast cancer.人类乳腺癌中的一氧化氮合酶活性
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8
Nitric oxide signaling to iron-regulatory protein: direct control of ferritin mRNA translation and transferrin receptor mRNA stability in transfected fibroblasts.一氧化氮向铁调节蛋白的信号传导：对转染成纤维细胞中铁蛋白mRNA翻译和转铁蛋白受体mRNA稳定性的直接控制。
Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1267-71. doi: 10.1073/pnas.92.5.1267.
9
Pteridine biosynthesis and nitric oxide synthase in Physarum polycephalum.多头绒泡菌中的蝶啶生物合成与一氧化氮合酶
Biochem J. 1994 Nov 15;304 ( Pt 1)(Pt 1):105-11. doi: 10.1042/bj3040105.
10
Translational regulation via iron-responsive elements by the nitric oxide/NO-synthase pathway.通过一氧化氮/一氧化氮合酶途径经由铁反应元件进行的翻译调控。
EMBO J. 1993 Sep;12(9):3651-7. doi: 10.1002/j.1460-2075.1993.tb06039.x.

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人子宫颈癌细胞系ME-180中钙/钙调蛋白依赖性一氧化氮合酶活性

Ca2+/calmodulin-dependent nitric oxide synthase activity in the human cervix carcinoma cell line ME-180.

作者信息

Werner-Felmayer G, Werner E R, Fuchs D, Hausen A, Mayer B, Reibnegger G, Weiss G, Wachter H

机构信息

Institute for Medical Chemistry and Biochemistry, University of Innsbruck, Austria.

出版信息

Biochem J. 1993 Jan 15;289 ( Pt 2)(Pt 2):357-61. doi: 10.1042/bj2890357.

DOI:10.1042/bj2890357
PMID:7678733
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1132175/
Abstract

We show here that the human cervix carcinoma cell line ME-180 expresses a constitutive nitric oxide (NO) synthase, as demonstrated by formation of [3H]citrulline and nitrite. The enzyme is dependent on tetrahydrobiopterin, NADPH, flavins and Ca2+/calmodulin. Enzyme activity is located in the cytosol rather than in the membrane fraction and can be inhibited by NG-monomethyl-L-arginine (NMMA). An antiserum to NO synthase purified from porcine cerebellum inhibited the enzyme activity. ME-180 cells released NO, as was shown by stimulation of guanylate cyclase (EC 4.6.1.2) in RFL-6 detector cells; this release was stimulated 8-fold by the Ca2+ ionophore A23187 and 2-fold by increasing the intracellular tetrahydrobiopterin levels with cytokines. This is the first characterization of a Ca2+/calmodulin-dependent NO synthase activity in human epithelial-type tumour cells.

摘要

我们在此表明，人宫颈癌细胞系ME-180表达一种组成型一氧化氮（NO）合酶，这通过[3H]瓜氨酸和亚硝酸盐的形成得以证明。该酶依赖于四氢生物蝶呤、NADPH、黄素和Ca2+/钙调蛋白。酶活性位于胞质溶胶而非膜组分中，并且可被NG-单甲基-L-精氨酸（NMMA）抑制。从猪小脑纯化的针对NO合酶的抗血清可抑制该酶活性。如在RFL-6检测细胞中鸟苷酸环化酶（EC 4.6.1.2）的刺激所显示，ME-180细胞释放NO；Ca2+离子载体A23187可使这种释放增加8倍，而细胞因子增加细胞内四氢生物蝶呤水平可使其增加2倍。这是首次对人上皮型肿瘤细胞中Ca2+/钙调蛋白依赖性NO合酶活性进行的表征。