Schmidt H H, Warner T D, Ishii K, Sheng H, Murad F
Department of Pharmacology, Northwestern University Medical School, Chicago, IL 60611.
Science. 1992 Feb 7;255(5045):721-3. doi: 10.1126/science.1371193.
L-arginine causes insulin release from pancreatic B cells. Data from three model systems support the hypothesis that L-arginine-derived nitrogen oxides (NOs) mediate insulin release stimulated by L-arginine in the presence of D-glucose and by the hypoglycemic drug tolbutamide. The formation of NO in pancreatic B cells was detected both chemically and by the NO-induced accumulation of guanosine 3',5'-monophosphate. NG-substituted L-arginine analogs inhibited the release of both insulin and NO. Protein immunoblot and histochemical analysis with antiserum to type I NO synthase suggest that the formation of NO in pancreatic B cells is catalyzed by an NADPH- (reduced form of nicotinamide adenine dinucleotide phosphate), Ca2+/calmodulin-dependent type I NO synthase of about 150 kilodaltons.
L-精氨酸可促使胰岛素从胰腺β细胞释放。来自三个模型系统的数据支持这样一种假说，即在存在D-葡萄糖和降糖药物甲苯磺丁脲的情况下，L-精氨酸衍生的氮氧化物（NOs）介导了由L-精氨酸刺激引起的胰岛素释放。通过化学方法以及NO诱导的3',5'-环磷酸鸟苷积累，检测到了胰腺β细胞中NO的形成。N-取代的L-精氨酸类似物抑制了胰岛素和NO的释放。用抗I型一氧化氮合酶抗血清进行的蛋白质免疫印迹和组织化学分析表明，胰腺β细胞中NO的形成是由一种约150千道尔顿的NADPH-（还原型烟酰胺腺嘌呤二核苷酸磷酸）、Ca2+/钙调蛋白依赖性I型一氧化氮合酶催化的。
