Dodd J G, Paraskevas M, McNicol P J
Department of Physiology, University of Manitoba, Winnipeg, Canada.
J Urol. 1993 Feb;149(2):400-2. doi: 10.1016/s0022-5347(17)36103-7.
Previously we demonstrated human papillomavirus type 16 DNA in a high proportion of benign hyperplastic (BPH) and cancerous (CaP) prostate specimens using the polymerase chain reaction (PCR). While these data designate the prostate as a possible reservoir for sexual transmission of HPV, an etiological role for the virus in prostatic neoplasia is uncertain. Since transcription of the E6/E7 genes of HPV 16 is essential for both viral replication and cellular transformation, we sought to assess the transcriptional activity of HPV 16 found in prostate tissues. The E6/E7 viral gene transcripts were identified in 5 of 10 BPH specimens and 3 of 7 CaP specimens known to contain HPV 16 DNA. Expression of the HPV viral genes is not associated preferentially with either BPH or CaP, nor is transcription observed in all samples which contain the viral genome. These findings suggest that the prostate may act as a site for HPV replication, but that HPV is unlikely to be involved in the transformation of prostatic cells.
此前，我们利用聚合酶链反应（PCR）在高比例的良性增生性（BPH）前列腺标本和癌性（CaP）前列腺标本中检测到16型人乳头瘤病毒（HPV）DNA。虽然这些数据表明前列腺可能是HPV性传播的一个潜在储存库，但该病毒在前列腺肿瘤形成中的病因学作用尚不确定。由于HPV 16的E6/E7基因转录对于病毒复制和细胞转化均至关重要，我们试图评估在前列腺组织中发现的HPV 16的转录活性。在已知含有HPV 16 DNA的10份BPH标本中的5份以及7份CaP标本中的3份中鉴定出了E6/E7病毒基因转录本。HPV病毒基因的表达并非优先与BPH或CaP相关，而且在所有含有病毒基因组的样本中均未观察到转录现象。这些发现表明，前列腺可能是HPV复制的一个场所，但HPV不太可能参与前列腺细胞的转化。
