Noda T, Sasagawa T, Dong Y, Fuse H, Namiki M, Inoue M
Department of Urology, Kanazawa University, School of Medicine, Ishikawa, Japan.
Urol Res. 1998;26(3):165-9. doi: 10.1007/s002400050041.
Human papillomavirus is thought to be an etiological factor for urological tumors such as penile cancer. However, there is much conflicting data surrounding prostatic cancer. We recently established a highly sensitive nested PCR method with consensus human papillomavirus (HPV) primers for the detection of many high-risk HPV types. HPV DNA from the long-control region (LCR) to E7 open reading frame was amplified with first primer pairs and subsequently amplified with second internal E6-E7 primers. Our nested PCR method could detect HPV16, 18, 31, 33, 35, 52, 58 and some undetermined HPV DNAs. Using this method, we investigated the existence of HPV DNA in formalin-fixed paraffin-embedded tissue of the prostate. We found HPV DNA in three of 71 specimens of benign prostatic hyperplasia (BPH) and in none of 38 prostatic carcinomas. These three samples were infected with HPV 16. These results suggest that HPV is not a causal factor for prostatic cancer and BPH.
人乳头瘤病毒被认为是阴茎癌等泌尿系统肿瘤的一个病因。然而，关于前列腺癌存在许多相互矛盾的数据。我们最近建立了一种高度灵敏的巢式PCR方法，使用人乳头瘤病毒（HPV）通用引物来检测多种高危HPV类型。从长控制区（LCR）到E7开放阅读框的HPV DNA先用第一对引物进行扩增，随后用第二对内部E6-E7引物进行扩增。我们的巢式PCR方法能够检测HPV16、18、31、33、35、52、58以及一些未确定的HPV DNA。利用该方法，我们研究了前列腺福尔马林固定石蜡包埋组织中HPV DNA的存在情况。我们在71例良性前列腺增生（BPH）标本中的3例中发现了HPV DNA，而在38例前列腺癌标本中均未发现。这三个样本感染的是HPV 16。这些结果表明，HPV不是前列腺癌和BPH的致病因素。
