Eizirik D L, Björklund A, Welsh N
Department of Medical Cell Biology, Uppsala University, Sweden.
FEBS Lett. 1993 Feb 8;317(1-2):62-6. doi: 10.1016/0014-5793(93)81492-i.
The cytokine interleukin 1 beta (IL-1 beta) induces the expression of an isoform of nitric oxide synthase (NOS) in insulin-producing cells which is similar to that expressed in activated macrophages. In the present study we show that IL-1 beta-induced expression of NOS mRNA in these cells is preceded by expression of c-fos mRNA. Moreover, the stimulatory effects of recombinant IL-1 beta on NOS mRNA expression are prevented by co-incubation with an inhibitor of gene transcription (actinomycin D) or an inhibitor of protein synthesis (cycloheximide). These data suggest that IL-1 beta-induced NOS mRNA expression may be mediated by transcription of immediate early response genes, and that c-fos may be one of these genes.
细胞因子白细胞介素1β（IL-1β）可诱导胰岛素生成细胞中一种一氧化氮合酶（NOS）同工型的表达，该同工型与活化巨噬细胞中表达的类似。在本研究中，我们发现这些细胞中IL-1β诱导的NOS mRNA表达之前会有c-fos mRNA的表达。此外，重组IL-1β对NOS mRNA表达的刺激作用可通过与基因转录抑制剂（放线菌素D）或蛋白质合成抑制剂（环己酰亚胺）共同孵育来阻断。这些数据表明，IL-1β诱导的NOS mRNA表达可能由即时早期反应基因的转录介导，且c-fos可能是这些基因之一。
