用水溶性聚合物探测短杆菌肽A通道。对通道状态电导的影响。
Probing alamethicin channels with water-soluble polymers. Effect on conductance of channel states.
作者信息
Bezrukov S M, Vodyanoy I
机构信息
University of Maryland, College Park 20742.
出版信息
Biophys J. 1993 Jan;64(1):16-25. doi: 10.1016/S0006-3495(93)81336-5.
Abstract
Channel access resistance has been measured to estimate the characteristic size of a single ion channel. We compare channel conductance in the presence of nonpenetrating water-soluble polymers with that obtained for polymer-free electrolyte solution. The contribution of the access resistance to the total alamethicin channel resistance is approximately 10% for first three open channel levels. The open alamethicin channel radii inferred for these first three levels from the access resistance are 6.3, 10.3, and 11.4 A. The dependence of channel conductance on polymer molecular weight also allows evaluation of the channel dimensions from polymer exclusion. Despite varying conductance, it was shown that steric radii of the alamethicin channel at different conductance levels remain approximately unchanged. These results support a model of the alamethicin channel as an array of closely packed parallel pores of nearly uniform diameter.
摘要
已测量通道接入电阻以估计单个离子通道的特征尺寸。我们将存在非穿透性水溶性聚合物时的通道电导与无聚合物电解质溶液的通道电导进行了比较。对于前三个开放通道水平，接入电阻对总短杆菌肽通道电阻的贡献约为10%。根据接入电阻推断出的前三个水平的开放短杆菌肽通道半径分别为6.3、10.3和11.4埃。通道电导对聚合物分子量的依赖性也允许从聚合物排除来评估通道尺寸。尽管电导不同，但结果表明，不同电导水平下短杆菌肽通道的空间半径大致保持不变。这些结果支持了短杆菌肽通道模型，即由一系列紧密排列的、直径几乎均匀的平行孔组成。
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