Jayaswal R K, Fernandez M, Upadhyay R S, Visintin L, Kurz M, Webb J, Rinehart K
Department of Biological Sciences, Illinois State University, Normal 61761.
Curr Microbiol. 1993 Jan;26(1):17-22. doi: 10.1007/BF01577237.
Two strains of Pseudomonas cepacia, RJ3 and ATCC 52796, have been identified as potential antagonists of fungal plant pathogens. We have compared the antagonistic activity of these two strains against various fungal pathogens. Although both strains displayed high levels of antagonism, ATCC 52796 was slightly more antagonistic than RJ3. The antagonist from RJ3 has been identified as the antifungal compound pyrrolnitrin after purification by HPLC and characterization by UV, IR, NMR, and mass spectroscopy. Both strains also antagonized the fungi by production of volatile compound(s), which have not yet been identified. Both strains are similar with respect to in vitro antagonism, mechanism of antagonism, and sensitivity to antibiotics.
洋葱伯克霍尔德菌的两个菌株RJ3和ATCC 52796已被鉴定为植物真菌病原体的潜在拮抗剂。我们比较了这两个菌株对各种真菌病原体的拮抗活性。虽然两个菌株都表现出高水平的拮抗作用，但ATCC 52796的拮抗作用略强于RJ3。通过高效液相色谱（HPLC）纯化并经紫外（UV）、红外（IR）、核磁共振（NMR）和质谱表征后，RJ3中的拮抗剂被鉴定为抗真菌化合物吡咯菌素。两个菌株还通过产生挥发性化合物来拮抗真菌，这些挥发性化合物尚未被鉴定。在体外拮抗作用、拮抗机制和对抗生素的敏感性方面，两个菌株相似。
