Brown J W, Haas E S, Pace N R
Department of Biology, Indiana University, Bloomington 47401.
Nucleic Acids Res. 1993 Feb 11;21(3):671-9. doi: 10.1093/nar/21.3.671.
The catalytic RNA component of bacterial RNase P is responsible for the removal of 5' leader sequences from precursor tRNAs. As part of an on-going phylogenetic comparative characterization of bacterial RNase P, the genes encoding RNase P RNA from the thermophiles Thermotoga maritima, Thermotoga neapolitana, Thermus aquaticus, and a mesophilic relative of the latter, Deinococcus radiodurans, have been cloned and sequenced. RNAs transcribed from these genes in vitro are catalytically active in the absence of other components. Active holoenzymes have been reconstituted from the T.aquaticus and T.maritima RNAs and the protein component of RNase P from Escherichia coli. The RNase P RNAs of T.aquaticus and T.martima, synthesized in vitro, were characterized biochemically and shown to be inherently resistant to thermal disruption. Several features of these RNAs suggest mechanisms contributing to thermostability. The new sequences provide correlations that refine the secondary structure model of bacterial RNase P RNA.
细菌核糖核酸酶P的催化RNA组分负责从前体转运RNA中去除5'前导序列。作为细菌核糖核酸酶P正在进行的系统发育比较表征的一部分，来自嗜热栖热菌、那不勒斯栖热菌、嗜热水生栖热菌以及后者的嗜温亲缘菌耐辐射球菌的核糖核酸酶P RNA编码基因已被克隆和测序。从这些基因体外转录的RNA在没有其他组分的情况下具有催化活性。已用嗜热水生栖热菌和嗜热栖热菌的RNA以及大肠杆菌核糖核酸酶P的蛋白质组分重建了活性全酶。体外合成的嗜热水生栖热菌和嗜热栖热菌的核糖核酸酶P RNA经生化表征，显示出对热破坏具有固有抗性。这些RNA的几个特征提示了有助于热稳定性的机制。新序列提供了相关信息，完善了细菌核糖核酸酶P RNA的二级结构模型。
