Miyamura Y, Tawa R, Koizumi A, Uehara Y, Kurishita A, Sakurai H, Kamiyama S, Ono T
Department of Radiation Research, Tohoku University School of Medicine, Sendai, Japan.
Mutat Res. 1993 Mar;295(2):63-9. doi: 10.1016/0921-8734(93)90002-k.
DNA methylation is known to change with age in several mammalian species. Here we have examined the effect of dietary energy restriction on this age-associated change in liver DNA of C3H/SHN mice. The total 5-methyldeoxycytidine level in the genome decreased slightly soon after energy restriction started. The effect, however, diminished with time and no appreciable difference was detected at middle and old ages. The degree of methylation at the c-myc gene, on the other hand, was not affected by energy restriction at early periods, but the age-dependent alterations at later ages were repressed. This is a new finding to show that DNA methylation is one of the molecular indices of aging affected by energy restriction. It suggests an importance of DNA methylation in the aging process.
已知在几种哺乳动物物种中，DNA甲基化会随着年龄的增长而发生变化。在此，我们研究了饮食能量限制对C3H/SHN小鼠肝脏DNA中这种与年龄相关变化的影响。能量限制开始后不久，基因组中总的5-甲基脱氧胞苷水平略有下降。然而，这种影响会随着时间减弱，在中年和老年时未检测到明显差异。另一方面，c-myc基因的甲基化程度在早期不受能量限制的影响，但在后期年龄依赖性变化受到抑制。这是一项新发现，表明DNA甲基化是受能量限制影响的衰老分子指标之一。它提示了DNA甲基化在衰老过程中的重要性。
