Ono T, Tawa R, Shinya K, Hirose S, Okada S
Biochem Biophys Res Commun. 1986 Sep 30;139(3):1299-304. doi: 10.1016/s0006-291x(86)80319-9.
The degree of methylation at c-myc proto-oncogene was found to change during aging process of mice by the use of methylation-sensitive restriction enzymes. The spleen DNA showed hypomethylation as mice aged, while hypermethylation was observed in the liver DNA. The brain DNA on the contrary revealed no appreciable difference between young and old mice. When the DNAs were examined at actin and dihydrofolate reductase (DHFR), no significant change was observed. It suggests that an age-related change of oncogene structure may be one of the factors which are related to an age-associated increase of cancer incidence rate.
通过使用甲基化敏感的限制性内切酶发现，在小鼠衰老过程中，c-myc原癌基因的甲基化程度发生了变化。随着小鼠年龄增长，脾脏DNA呈现低甲基化，而肝脏DNA中观察到高甲基化。相反，脑DNA在年轻和年老小鼠之间未显示出明显差异。当检测肌动蛋白和二氢叶酸还原酶（DHFR）的DNA时，未观察到显著变化。这表明癌基因结构的年龄相关变化可能是与年龄相关的癌症发病率增加相关的因素之一。
