Takeda N, Kalubi B, Abe Y, Irifune M, Ogino S, Matsunaga T
Department of Otolaryngology, Osaka University Medical School, Japan.
Acta Otolaryngol Suppl. 1993;501:21-4. doi: 10.3109/00016489309126207.
The role of neuropeptides in nasal allergy was examined in guinea pigs by histochemical and pharmacological study. Intranasal application of toluene diisocyanate (TDI) induced nasal allergy-like behaviors: sneezing and watery rhinorrhea, and decreased histamine content in the nasal mucosa in guinea pigs sensitized with TDI. The immunoreactivity of substance P (SP) and calcitonin gene-related peptide (CGRP) in the nerve terminals in the nasal mucosa was increased after intranasal application of TDI. We also observed a decrease in the immunoreactivity of SP and CGRP, and an increase in their mRNA expression in the trigeminal ganglion neurons. These findings indicate that exposure to TDI enhanced the biosynthesis of both SP and CGRP in the trigeminal ganglion neurons and their axonal transportation to the terminals in the nasal mucosa. In animals pretreated with capsaicin before sensitization, TDI did not induce nasal allergy-like behaviors and histamine release in the nasal mucosa. Since capsaicin depletes SP and CGRP in the sensory nerves, this finding indicates neuropeptide-mediated histamine release in the nasal mucosa. All these findings suggest that, on exposure to TDI, the antidromic release of SP and CGRP in the nasal mucosa triggers the release of histamine, resulting in the development of symptoms of nasal allergy.
通过组织化学和药理学研究，在豚鼠中检测了神经肽在鼻过敏中的作用。鼻内应用甲苯二异氰酸酯（TDI）可诱发类似鼻过敏的行为：打喷嚏和水样鼻溢，并降低了用TDI致敏的豚鼠鼻黏膜中的组胺含量。鼻内应用TDI后，鼻黏膜神经末梢中P物质（SP）和降钙素基因相关肽（CGRP）的免疫反应性增强。我们还观察到三叉神经节神经元中SP和CGRP的免疫反应性降低，而它们的mRNA表达增加。这些发现表明，暴露于TDI可增强三叉神经节神经元中SP和CGRP的生物合成及其向鼻黏膜神经末梢的轴突运输。在致敏前用辣椒素预处理的动物中，TDI未诱发类似鼻过敏的行为和鼻黏膜中的组胺释放。由于辣椒素会耗尽感觉神经中的SP和CGRP，这一发现表明神经肽介导鼻黏膜中的组胺释放。所有这些发现表明，暴露于TDI时，鼻黏膜中SP和CGRP的逆向释放会触发组胺释放，从而导致鼻过敏症状的出现。
