Doughty M J, Nguyen K T
University of Waterloo, School of Optometry, Ont., Canada.
Ophthalmic Res. 1995 Mar-Apr;27(2):80-8. doi: 10.1159/000267602.
Corneal stroma-endothelial preparations from 2-kg albino rabbits were incubated in vitro in a bicarbonate-Ringer solution equilibrated with 5% CO2-9% O2 over 4 h in half-chambers at 37 degrees C. An average net fluid pump activity of 6.8 microliters/h was measured in the 4th hour. If such preparations were initially equilibrated with bicarbonate-Ringer for 90 min and then challenged for 20 min with 15 mM sodium lactate or NaCl, an osmotically induced increase in fluid flow occurred. After removal of the challenge and return to bicarbonate-Ringer for 2.5 h, the net fluid pump returned to baseline and then increased again (to an average of 9.7 microliters/h) for the lactate- but not NaCl-challenged endothelia. Scanning electron microscopy showed that lactate-challenged (but not the control or NaCl-challenged) endothelial contained a moderate number of very large pleomorphic cells within an otherwise regular mosaic. It is proposed that the large cells develop as a result of cell fusion (coalescence).
取体重2千克的白化兔的角膜基质 - 内皮制剂，于37摄氏度在半透室中，在与5%二氧化碳 - 9%氧气平衡的碳酸氢盐 - 林格氏溶液中体外孵育4小时。在第4小时测得平均净液体泵活性为6.8微升/小时。如果此类制剂最初用碳酸氢盐 - 林格氏液平衡90分钟，然后用15毫摩尔乳酸钠或氯化钠刺激20分钟，会出现渗透压诱导的液体流动增加。去除刺激并回到碳酸氢盐 - 林格氏液2.5小时后，净液体泵恢复到基线水平，然后对于乳酸刺激而非氯化钠刺激的内皮再次增加（平均达到9.7微升/小时）。扫描电子显微镜显示，乳酸刺激的内皮（而非对照或氯化钠刺激的内皮）在规则的镶嵌结构中含有数量适中的非常大的多形细胞。据推测，大细胞是细胞融合（聚结）的结果。
