Ratcliffe A, Shurety W, Caterson B
Department of Orthopaedic Surgery, Columbia University, New York, New York.
Arthritis Rheum. 1993 Apr;36(4):543-51. doi: 10.1002/art.1780360416.
Previous studies have shown the presence of a native chondroitin sulfate epitope in articular cartilage proteoglycans from canine knee joints with experimental early osteoarthritis (OA), but not in normal cartilage. The objective of this study was to quantitate the native epitope recognized by monoclonal antibody 3-B-3 in synovial fluids and articular cartilage of diseased joints.
An immunoassay with monoclonal antibody 3-B-3, which recognizes a native chondroitin-6-sulfate structure, was developed and used to analyze synovial fluid lavage material and extracts of articular cartilage from canine knee joints with early experimental OA or with mild disuse atrophy, and from control animals.
The concentration of epitope in the OA fluids was elevated 33-35-fold, and in the OA articular cartilage extracts it was elevated > 200-fold, compared with samples from the control group. No significant difference was detected in the levels of 3-B-3 epitope in the synovial fluid lavage material or cartilage extracts from the joints of the disuse group versus the control group.
The native 3-B-3 epitope in articular cartilage and synovial fluids may be a specific marker of ongoing anabolic events in early degenerative joint disease.
先前的研究表明，在患有实验性早期骨关节炎（OA）的犬膝关节的关节软骨蛋白聚糖中存在一种天然硫酸软骨素表位，但在正常软骨中不存在。本研究的目的是定量单克隆抗体3 - B - 3在患病关节的滑液和关节软骨中识别的天然表位。
开发了一种使用单克隆抗体3 - B - 3的免疫测定法，该抗体识别天然硫酸软骨素-6-硫酸酯结构，并用于分析来自患有早期实验性OA或轻度废用性萎缩的犬膝关节以及对照动物的关节软骨提取物的滑液灌洗材料。
与对照组样本相比，OA滑液中表位浓度升高了33 - 35倍，OA关节软骨提取物中表位浓度升高了200倍以上。在废用组关节的滑液灌洗材料或软骨提取物中，未检测到3 - B - 3表位水平与对照组有显著差异。
关节软骨和滑液中的天然3 - B - 3表位可能是早期退行性关节疾病中正在进行的合成代谢事件的特异性标志物。
