Sandvej K B, Hamilton-Dutoit S J, Pallesen G
Laboratory of Immunohistology, Aarhus University Hospital, Denmark.
Leuk Lymphoma. 1993 Jan;9(1-2):95-101. doi: 10.3109/10428199309148510.
Epstein-Barr virus (EBV) latent membrane protein 1 (LMP 1) is expressed in Hodgkin and Reed-Sternberg (HRS) cells in about one half of Hodgkin's disease (HD) cases. In vitro, LMP 1 induces B-cell expression of CD23 antigen, ICAM-1 and LFA-3. To evaluate the influence of LMP 1 on the expression of these molecules in HRS cells in vivo, we performed a quantitative frozen section immunohistological study comparing the numerical density (cells per unit area) of HRS cells expressing the CD23 antigen, ICAM-1 and LFA-3 in 14 LMP 1-positive and 13 LMP 1-negative HD cases. CD23 antigen was demonstrated in HRS cells in five LMP 1-positive and three LMP 1-negative cases (not significant). The relative density of HRS cells tended to be lower in the LMP 1-positive than in the LMP 1-negative cases, but this did not reach significance (0.2 > 2p > 0.1). All recognizable HRS cells expressed ICAM-1 and LFA-3 irrespective of LMP 1 status. We conclude that expression of CD23 antigen and LMP 1 are not coordinated in HD. Although LMP 1 may have some influence on CD23 antigen expression, it is unlikely that the latter is of importance in the putative EBV induced growth transformation of HRS cells in vivo.
在大约一半的霍奇金淋巴瘤（HD）病例中，霍奇金和里德-斯腾伯格（HRS）细胞中可表达爱泼斯坦-巴尔病毒（EBV）潜伏膜蛋白1（LMP 1）。在体外，LMP 1可诱导B细胞表达CD23抗原、细胞间黏附分子-1（ICAM-1）和淋巴细胞功能相关抗原-3（LFA-3）。为评估LMP 1对体内HRS细胞中这些分子表达的影响，我们进行了一项定量冰冻切片免疫组织学研究，比较了14例LMP 1阳性和13例LMP 1阴性HD病例中表达CD23抗原、ICAM-1和LFA-3的HRS细胞的数量密度（每单位面积的细胞数）。在5例LMP 1阳性和3例LMP 1阴性病例的HRS细胞中检测到了CD23抗原（无显著性差异）。LMP 1阳性病例中HRS细胞的相对密度往往低于LMP 1阴性病例，但未达到显著性差异（0.2＞P＞0.1）。无论LMP 1状态如何，所有可识别的HRS细胞均表达ICAM-1和LFA-3。我们得出结论，在HD中CD23抗原和LMP 1的表达不协调。尽管LMP 1可能对CD23抗原表达有一定影响，但在体内推测的EBV诱导的HRS细胞生长转化过程中，后者不太可能起重要作用。
