Tao Q, Srivastava G, Loke S L, Ho F C
Department of Pathology, Queen Mary Hospital, University of Hong Kong.
J Clin Pathol. 1994 Jul;47(7):589-91. doi: 10.1136/jcp.47.7.589.
To evaluate whether there is any correlation between the expression of Epstein-Barr virus (EBV) latent membrane protein (LMP) and oncoprotein bcl-2 in the lymph node biopsy specimens of a Chinese patient with EBV-related reactive lymphoproliferation who later developed T cell lymphoma after a short period of time.
Immunohistochemistry, with a standard alkaline phosphatase antialkaline phosphatase (APAAP) method and New Fuchsin as a chromogen, was used for single staining of bcl-2 or LMP. Double immunostaining combining APAAP and indirect immunofluorescence was performed for dual labelling of LMP and bcl-2.
bcl-2 was expressed in 10-30% of cells in the first lymph node biopsy specimen (EBV-associated lymphoproliferative disorder) and 30-50% of cells in the second lymph node biopsy specimen (T cell lymphoma). LMP was expressed in the first biopsy specimen but not in the second. Double immunostaining results showed that around 78% of LMP positive cells were bcl-2 negative and 94% bcl-2 positive cells were LMP negative. Among the very small fraction of LMP and bcl-2 double positive cells, the intensity of bcl-2 staining was heterogeneous and was not always stronger than that observed in LMP negative bcl-2 positive cells.
The expression of bcl-2 protein is independent of LMP protein status in vivo. Several mechanisms may be involved in EBV associated lymphomagenesis, and bcl-2 induction may occur independently of LMP expression.
评估一名患有爱泼斯坦-巴尔病毒（EBV）相关反应性淋巴细胞增生的中国患者的淋巴结活检标本中，EBV潜伏膜蛋白（LMP）表达与癌蛋白bcl-2之间是否存在相关性，该患者在短时间后发展为T细胞淋巴瘤。
采用标准碱性磷酸酶抗碱性磷酸酶（APAAP）法，以新福林作为显色剂，进行免疫组织化学单染bcl-2或LMP。采用结合APAAP和间接免疫荧光的双重免疫染色对LMP和bcl-2进行双标记。
在首次淋巴结活检标本（EBV相关淋巴细胞增生性疾病）中，10%-30%的细胞表达bcl-2；在第二次淋巴结活检标本（T细胞淋巴瘤）中，30%-50%的细胞表达bcl-2。LMP在首次活检标本中表达，但在第二次活检标本中未表达。双重免疫染色结果显示，约78%的LMP阳性细胞bcl-2阴性，94%的bcl-2阳性细胞LMP阴性。在极少部分LMP和bcl-2双阳性细胞中，bcl-2染色强度不均一，并不总是强于LMP阴性bcl-2阳性细胞中的染色强度。
体内bcl-2蛋白的表达独立于LMP蛋白状态。EBV相关淋巴瘤发生可能涉及多种机制，bcl-2的诱导可能独立于LMP表达而发生。
