用赖氨酸取代异亮氨酸364可阻断白喉毒素的膜转位和通道形成活性。
Membrane translocation and channel-forming activities of diphtheria toxin are blocked by replacing isoleucine 364 with lysine.
作者信息
Cabiaux V, Mindell J, Collier R J
机构信息
Laboratoire des Macromolécules aux Interfaces, Université Libre de Bruxelles.
出版信息
Infect Immun. 1993 May;61(5):2200-2. doi: 10.1128/iai.61.5.2200-2202.1993.
Abstract
A mutant of diphtheria toxin in which Ile-364 was replaced by Lys was at least 500-fold less toxic to Vero cells than the parental toxin. Its ability to undergo low-pH-triggered translocation across the plasma membrane was greatly diminished, as was its ability to form ion-conductive channels. In addition, the mutant toxin was inactive in the pH-dependent killing of Escherichia coli.
摘要
一种将异亮氨酸-364替换为赖氨酸的白喉毒素突变体对非洲绿猴肾细胞（Vero细胞）的毒性比亲本毒素至少低500倍。其在低pH触发下跨质膜转运的能力大大降低，形成离子传导通道的能力也同样如此。此外，该突变毒素在对大肠杆菌的pH依赖性杀伤中无活性。
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