Swerdlow S H, Westermann C D, Pelstring R J, Saboorian M H, Williams M E
Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, OH.
Hum Pathol. 1993 May;24(5):540-6. doi: 10.1016/0046-8177(93)90167-f.
Measurement of growth fraction is an important way to provide an objective assessment of non-Hodgkin's lymphomas; however, many of the techniques used require fresh tissue and/or special instrumentation. Recently, antibodies to the proliferating cell nuclear antigen (PCNA)/cyclin reactive in paraffin-embedded sections have become available. To investigate the utility of one such antibody in the study of follicular center cell (FCC) lymphomas and cleaved cell lymphomas of centrocytic type (CC), paraffin sections from 40 cases that had been characterized in two previous morphometric studies were stained with a PCNA antibody. Strong correlations were found between PCNA staining in formalin- and B5-fixed tissues, between the overall proportion of PCNA-positive cells and the proportion in the area of greatest staining, and between strong and total staining. Proliferating cell nuclear antigen staining was significantly stronger in the noncleaved FCC lymphomas than in the cleaved cell lymphomas. The FCC lymphomas showed moderate to strong correlations between PCNA staining and morphometric features of transformation, but only nuclear area correlated with PCNA staining in the CC group. Proliferating cell nuclear antigen staining was not significantly different between CC lymphomas with and without the characteristic bcl-1/PRAD 1 gene rearrangement. In summary, PCNA staining of either B5- or formalin-fixed, paraffin-embedded tissue sections is a simple aid in the objective categorization of FCC lymphomas and may offer additional potentially prognostic information in some FCC subsets and in CC lymphomas. The findings further support the distinction between CC and true FCC lymphomas.
测量生长分数是客观评估非霍奇金淋巴瘤的重要方法；然而，许多所使用的技术需要新鲜组织和/或特殊仪器。最近，可用于石蜡包埋切片中增殖细胞核抗原（PCNA）/细胞周期蛋白反应的抗体已经出现。为了研究一种此类抗体在滤泡中心细胞（FCC）淋巴瘤和中心细胞型（CC）裂细胞淋巴瘤研究中的效用，用PCNA抗体对来自40例病例的石蜡切片进行染色，这些病例在之前的两项形态计量学研究中已被特征化。在福尔马林固定和B5固定组织中的PCNA染色之间、PCNA阳性细胞的总体比例与最大染色区域中的比例之间以及强染色和总染色之间发现了强相关性。非裂FCC淋巴瘤中的增殖细胞核抗原染色明显强于裂细胞淋巴瘤。FCC淋巴瘤在PCNA染色与转化的形态计量学特征之间显示出中度至强相关性，但在CC组中只有核面积与PCNA染色相关。有和没有特征性bcl-1/PRAD 1基因重排的CC淋巴瘤之间的增殖细胞核抗原染色没有显著差异。总之，对B5或福尔马林固定、石蜡包埋的组织切片进行PCNA染色有助于对FCC淋巴瘤进行客观分类，并且可能在一些FCC亚组和CC淋巴瘤中提供额外的潜在预后信息。这些发现进一步支持了CC淋巴瘤与真正的FCC淋巴瘤之间的区别。
