用于tRNA-酶识别的镶嵌瓷砖模型。
Mosaic tile model for tRNA-enzyme recognition.
作者信息
Steinberg S V, Kisselev L L
机构信息
Engelhardt Institute of Molecular Biology, Moscow, Russia.
出版信息
Nucleic Acids Res. 1993 Apr 25;21(8):1941-7. doi: 10.1093/nar/21.8.1941.
An improved algorithm was elaborated to analyse tRNA interaction with aminoacyl-tRNA synthetase based on analysis of tRNA sequences. The fundamental element defining the interaction between the tRNA and the synthetase is not a single nucleotide but a nucleotide combination named a tile which comprises of a given nucleotide and its neighbours as they are defined by the tertiary structure of the molecule. Informational content of each tile is calculated as its probability to occur exclusively in a set of cognate tRNAs. Based on this algorithm the identity sites of E. coli tRNA(Ala) and tRNA(Gln) were determined. The results are in a good agreement with the biochemical data and provide new information about identity sites of these tRNAs.
基于对tRNA序列的分析，精心设计了一种改进算法，用于分析tRNA与氨酰tRNA合成酶的相互作用。决定tRNA与合成酶之间相互作用的基本要素不是单个核苷酸，而是一种名为“tile”的核苷酸组合，它由给定核苷酸及其相邻核苷酸组成，这些核苷酸由分子的三级结构确定。每个tile的信息含量通过其仅在一组同源tRNA中出现的概率来计算。基于该算法，确定了大肠杆菌tRNA(Ala)和tRNA(Gln)的识别位点。结果与生化数据高度吻合，并提供了有关这些tRNA识别位点的新信息。
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