改良的酸性硫氰酸胍-酚-氯仿RNA提取方法，可大大减少DNA污染。 - Suppr

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超能文献

Modified acid guanidinium thiocyanate-phenol-chloroform RNA extraction method which greatly reduces DNA contamination.

作者信息

Siebert P D, Chenchik A

机构信息

CLONTECH Laboratories, Inc., Palo Alto, CA 94303-4607.

出版信息

Nucleic Acids Res. 1993 Apr 25;21(8):2019-20. doi: 10.1093/nar/21.8.2019.

DOI:10.1093/nar/21.8.2019

PMID:7684133

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC309453/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e5f/309453/527d0aaa75a7/nar00057-0325-a.jpg

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Modified acid guanidinium thiocyanate-phenol-chloroform RNA extraction method which greatly reduces DNA contamination.改良的酸性硫氰酸胍-酚-氯仿RNA提取方法，可大大减少DNA污染。

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本文引用的文献

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.采用酸性硫氰酸胍-苯酚-氯仿萃取法一步分离RNA的方法。

Anal Biochem. 1987 Apr;162(1):156-9. doi: 10.1006/abio.1987.9999.

Use of reverse transcriptase polymerase chain reaction to monitor expression of intronless genes.使用逆转录聚合酶链反应监测无内含子基因的表达。

Biotechniques. 1990 Sep;9(3):262, 264, 266-8.

Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.从富含核糖核酸酶的来源中分离生物活性核糖核酸。

Biochemistry. 1979 Nov 27;18(24):5294-9. doi: 10.1021/bi00591a005.

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